PMID: 6162483Jan 20, 1981Paper

Measurement of ribonucleotide pool specific activities by an in vivo method: comparison with an in vitro method

Biochemistry
S Falkenthal, J A Lengyel

Abstract

A biological assay is described for the specific activity of the nucleotides incorporated into nuclear ribonucleic acid (RNA). Under labeling conditions in which the pool specific activity reaches steady state by a process described by a continuous function, this method allows the determination of pool specific activity at all times of labeling. In Drosophila cultured cells incubated with [3H]uridine, the incorporation of radioactivity into transfer RNA (tRNA) and the absolute rate of tRNA synthesis were used to obtain an absolute value for the average specific activities of the uridine 5'-triphosphate (UTP) and cytidine 5'-triphosphate (CTP) precursor pools. The average specific activity of the UTP and CTP pool obtained by this in vivo method was 2-3 fold higher than the specific activity of the whole-cell UTP and CTP pools as assayed by an in vitro method. This suggests that the ribonucleotide pools in Drosophila cultured cells are compartmentalized. Use of the in vivo assay of ribonucleotide pool specific activities described here should avoid underestimates or overestimates which may arise when absolute rates of RNA synthesis are calculated from measurements of whole-cell ribonucleotide pool specific activities. The rate of...Continue Reading

References

Feb 1, 1979·Nucleic Acids Research·S SilvermanU L RajBhandary
Feb 1, 1979·Nucleic Acids Research·S SilvermanD Söll
May 1, 1979·Developmental Biology·K V Anderson, J A Lengyel
Jan 1, 1975·Methods in Cell Biology·J LengyelS Penman
Mar 1, 1977·Journal of Cellular Physiology·L Puckett, J E Darnell
Jun 1, 1977·Developmental Biology·J A Lengyel, S Penman
Jun 15, 1973·Journal of Molecular Biology·R Soeiro, E Ehrenfeld
Nov 5, 1973·Journal of Molecular Biology·M Hirsch, S Penman
Nov 16, 1973·Biochemical and Biophysical Research Communications·G KramerH Hilz
Jun 14, 1971·Journal of Molecular Biology·R S Wu, R Soeiro

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Citations

Jan 1, 1988·The International Journal of Biochemistry·M AnderssonU Stenram
Jan 1, 1985·Bio Systems·P M Bhargava
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffD Patterson
Feb 1, 1981·Developmental Biology·K V Anderson, J A Lengyel
Oct 1, 1981·Analytical Biochemistry·A L PogolottiD V Santi
Jan 1, 1985·CRC Critical Reviews in Biochemistry·J D Moyer, J F Henderson

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