PMID: 8608198Oct 1, 1995Paper

Measurement of S-100 protein in human blood and cerebrospinal fluid: analytical method and preliminary clinical results

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
Ulrich Missler, Martin Wiesmann

Abstract

An immunofluorometric sandwich assay for determination of S-100 protein in cerebrospinal fluid (CSF) and blood is described. The lower detection limit was 0.015 micrograms/l of S-100 protein. Intra-assay and inter-assay imprecision (coefficients of variation, CVs) were 2.1 to 3.2% and 7.8 to 11.6%, respectively. S-100 protein recovery in cerebrospinal fluid was 94 to 103%. In blood the recovery varied from 67 to 96%, depending on blood samples used and the concentration of S-100 protein. The best recovery in blood was found using heparinized plasma. In healthy subjects 0.098 +/- 0.11 micrograms/l (mean +/- SD) of S-100 protein was detected (n = 30). In the CSF of otherwise healthy patients undergoing a myelography for lumbar pain 1.43 +/- 0.49 micrograms/l (mean +/- SD) of S-100 protein was found. Preliminary results from longitudinal studies on S-100 protein in neurosurgical patients indicate a positive correlation between S-100 protein blood levels and clinical course. Thus, the determination of S-100 protein in blood appears to be helpful in the monitoring of patients with neuronal damage.

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Citations

Jan 1, 1997·Acta neurochirurgica·M WiesmannD Gottmann
Jan 1, 1997·Acta neurochirurgica·C WoertgenA Brawanski
Jan 5, 2000·The Annals of Thoracic Surgery·A EttingerA Y Razumovsky
Sep 5, 2001·Journal of Affective Disorders·M RothermundtH Kirchner
Sep 22, 2005·Journal of Neurology, Neurosurgery, and Psychiatry·C FoerchM Sitzer
Jul 4, 2007·Journal of Neuroinflammation·Anthony J WilliamsFrank C Tortella
Jan 1, 2012·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Chung-Hsiang LiuChing-Liang Hsieh
Aug 24, 2010·Progress in Neuro-psychopharmacology & Biological Psychiatry·Kai-Ren LuoShih-Jen Tsai
Mar 21, 2006·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Nico J M van BeverenDurk Fekkes

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