Measurement of serum and plasma osmolality in healthy young humans--influence of time and storage conditions

Clinical Chemistry and Laboratory Medicine : CCLM
Christian C SeifarthJohannes Hensen

Abstract

The precise measurement of osmolality is crucial in the differential diagnosis of disorders of water balance. Storage conditions, and freezing and thawing of serum or plasma samples before osmometry may influence the accuracy of measured values. A series of serum and plasma samples of 25 healthy young individuals were stored under different conditions at different temperatures (room temperature (22 degrees C), 7 degrees C, -21 degrees C, -78 degrees C) for up to 56 days. Before freezing a protein-stabilizing agent (bacitracin) was added to one part of the samples. Osmolality was examined using the freezing point method. At room temperature osmolality was stable for up to 3 days but showed a tendency toward an increase that was significant on day 14. In contrast, at 7 degrees C an initial significant decrease in serum osmolality occurred (day 1), which was followed by a slow increase. Serum samples stored at -21 degrees C showed a significantly lower osmolality on the 14th day compared to baseline. Adding bacitracin before freezing reduced this decrease by more than half, but the deviation was still significant. In samples stored at -78 degrees C no significant alteration of osmolality from baseline was observed over the observa...Continue Reading

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Citations

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