Measurement of serum nitrite/nitrate concentrations using high-performance liquid chromatography.

Journal of Chromatography. B, Biomedical Sciences and Applications
I El MenyawiH Burgmann

Abstract

Previous studies have reported increased serum concentrations of nitrite/nitrate - the degradation products of nitric oxide - in Plasmodium vivax malaria and uncomplicated Plasmodium falciparum malaria. In all these studies, however, nitrite/nitrate has been measured spectrometrically using Griess reagent which carries major disadvantages in the determination of serum nitrite/nitrate. The method does not allow an exact differentiation of nitrite and biogenic amines that are physiologically present in plasma. In the present study we introduce high-performance liquid chromatography as a new, accurate and cost effective method for determination of serum nitrite/nitrate levels. Significantly increased nitrate concentrations were found in malaria patients and serum values remained above normal levels for at least 21 days. It could be shown that our HPLC method is a sensitive and cost-effective method for direct determination of nitrite/nitrate in serum samples, which is not influenced by the presence of biogenic amines.

References

Jun 1, 1994·The Journal of Infectious Diseases·S CotW M Eling

❮ Previous
Next ❯

Citations

Jan 11, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·P LicataG mo Dugo
Jan 5, 2000·Clinical and Diagnostic Laboratory Immunology·B StoiserH Burgmann
Aug 30, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mohammad Reza GanjaliMasoud Salavati-Niasari
Aug 15, 2006·Nitric Oxide : Biology and Chemistry·Stefan WinklerClaus G Krenn
Aug 29, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·O W SakowitzA W Unterberg
Dec 23, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irina C ChisSimona Clichici
Jun 29, 1999·Analytical Chemistry·D J Anderson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.