Measurement of sex steroid hormones in breast adipocytes: methods and implications.

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
Roni T FalkMark E Sherman

Abstract

The lack of validated methods for measuring sex steroid hormones in breast tissue has limited our knowledge of their role in the development of breast cancer. We explored the feasibility of measuring hormones in breast adipocytes for epidemiologic and clinical studies by refining an existing assay procedure and assessing the reliability of hormone measurements using the modified assay. This report presents the reproducibility of measurements of androstenedione (A), testosterone (T), estrone (E(1)), and estradiol (E(2)), using breast adipose tissue samples obtained from women undergoing surgical resection for a variety of pathologic conditions. Breast adipose tissues were obtained from 20 women. Measurements of the steroid hormones were carried out by harvesting oil from adipocytes following enzymatic digestion of the adipose tissue, extracting and chromatographing the steroids, and quantifying them by RIA. The study was conducted in three phases: first, samples from five women were used to assess the assay procedure; following this, tissues from an additional five women were assayed repeatedly to determine reproducibility of the hormone measurements. Finally, using samples from 10 women undergoing surgical resection of a breast...Continue Reading

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Citations

Aug 27, 2011·Breast Cancer Research and Treatment·Roni T FalkMark E Sherman
Apr 7, 2010·Science Translational Medicine·Noha A MousaRobert F Casper
May 21, 2010·Molecular Nutrition & Food Research·Selin BolcaHerman Depypere
Apr 22, 2015·Analytical Chemistry·Jihye KimAaron R Wheeler
Oct 2, 2013·The Journal of Clinical Endocrinology and Metabolism·Feng WangMatti J Tikkanen
Feb 19, 2010·The American Journal of Clinical Nutrition·Selin BolcaHerman Depypere
Aug 30, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Lauren CarlsonNatalie D Shaw
Jun 23, 2012·The Journal of Clinical Endocrinology and Metabolism·Feng WangMatti J Tikkanen
Jan 21, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ju-Yeon MoonMan Ho Choi
Jun 3, 2021·International Journal of Molecular Sciences·Marcela Valko-RokytovskáZuzana Kostecká

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