Measurement of soluble and membrane-bound interleukin 1 using a fibroblast bioassay

Current Protocols in Immunology
Charles A DinarelloS K Durum

Abstract

Interleukin 1 (IL-1) is both a major mediator of inflammation and an important signal for activation and differentiation of lymphoid cells. IL-1 is produced by a large variety of cell types, but in most immunologic systems, activated macrophages are its major cellular source. This unit describes a bioassay for testing biological fluids based on the ability of IL-1 to induce IL-8 or IL-6 in fibroblasts. Also listed are sources of commercially available kits (ELISAs, immunoassays) to measure IL-1, and antibodies useful in setting up these assays.

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Citations

Aug 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hajime KonoKenneth L Rock

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