Measurement of surface concentration of fluorophores by fluorescence fluctuation spectroscopy

Optics Letters
A DelonR Jaffiol

Abstract

Fluorescence fluctuation spectroscopy is applied to study molecules passing through a small observation volume, usually subjected to diffusive or convective motion in a liquid phase. We suggest that such a technique could be used to measure the areal absolute concentration of fluorophores deposited on a substrate or embedded in a thin film, with a resolution of a few micrometers. The principle is to translate the solid substrate in front of a confocal fluorescence microscope objective and to record the subsequent fluctuations of the fluorescence intensity. The validity of this concept is investigated on model substrates (fluorescent microspheres) and DNA biochips.

References

Jul 2, 1999·Biophysical Journal·Y ChenE Gratton
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P KaskK Gall
Mar 12, 2004·Annual Review of Physical Chemistry·X S Xie, J K Trautman
Jun 11, 2004·Biophysical Journal·Joachim D Müller
May 17, 2005·Biophysical Journal·Joseph P SkinnerJoachim D Müller

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Citations

Jan 12, 2011·Annual Review of Physical Chemistry·Hai-Tao He, Didier Marguet

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