Measurement of T and B cell turnover with bromodeoxyuridine

Current Protocols in Immunology
David F ToughGeoffrey L Stephens

Abstract

This unit describes two methods used to determine the rate of turnover of T and B cells in vivo. In each method, this is accomplished by administering 5-bromo-2'-deoxyuridine (BrdU), a thymidine analog, and measuring the rate at which cells become labeled with this DNA precursor during the S phase of the cell cycle. Cells that contain BrdU are then detected in vitro using fluorochrome-labelled anti-BrdU monoclonal antibody by flow cytometry. The unit includes an Alternate Protocol that may be useful in situations where the Basic Protocol proves incompatible with particular antibody-fluorochrome conjugates or other downstream staining procedures. Using this procedure, a wide range of antibody-fluorochrome combinations can be used.

References

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Citations

Sep 5, 2013·Infection and Immunity·Michael M OpataBeth A Garvy
Jan 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy M PastvaJo Rae Wright
Jan 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sambuddho MukherjeeJo Rae Wright
Nov 20, 2016·European Journal of Immunology·Fatima ManzoorRoland Tisch

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