Measurement of the 4,834-bp mitochondrial DNA deletion level in aging rat liver and brain subjected or not to caloric restriction diet

Annals of the New York Academy of Sciences
P CassanoMaria Nicola Gadaleta

Abstract

Several studies have demonstrated an age-related accumulation of the amount of a specific 4834-bp mitochondrial DNA (mtDNA) deletion in different tissues of rat (liver, brain, and skeletal muscle). We investigated the influence of a caloric restriction diet (CR) on a selected age-associated marker of mtDNA damage, as the 4834-bp deletion, using quantitative real-time PCR. The mtDNA deleted level has been determined with respect to the mitochondrial D-loop level, using specific primers and TaqMan probes for each target. In liver we found an age-related increase of the deletion level (twofold) that was reversed and brought back to the adult level by a CR diet. On the contrary, in the brain the age-related increase of the deletion level (eightfold) was not affected by CR at all. The different effect of the CR on the deletion level in liver and brain might be a further element supporting the tissue-specificity of the aging process.

References

Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C RichterB N Ames
Jun 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L E AspnesJ M Aiken
Jan 22, 1998·Free Radical Biology & Medicine·C M KangB P Yu
May 25, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A M LezzaM N Gadaleta
Jul 11, 2000·Nature Genetics·C K LeeT A Prolla
Jul 12, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry Drew, Christiaan Leeuwenburgh

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Citations

Oct 19, 2007·Zeitschrift für Gerontologie und Geriatrie·C Meissner
Feb 3, 2009·Journal of Toxicology and Environmental Health. Part a·Mohammed AzabKarem Alzoubi
Sep 8, 2005·Rejuvenation Research·Rafal M Smigrodzki, Shaharyar M Khan
May 19, 2006·Rejuvenation Research·P CassanoM N Gadaleta
Dec 7, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Christy S CarterChristian Leeuwenburgh
Feb 27, 2007·PLoS Genetics·Gregory C KujothTomas A Prolla
Jan 15, 2011·Journal of Aging Research·Ricardo Gredilla
Jun 16, 2011·Journal of Proteomics·Clara MusiccoMaria Nicola Gadaleta
May 12, 2009·Mechanisms of Ageing and Development·Simone C McInernyDoug W Smith
May 17, 2005·Mechanisms of Ageing and Development·David A Sinclair
Jun 29, 2016·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Alan DiotJoanna Poulton

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