PMID: 6412757Sep 28, 1983Paper

Measurement of the amount of ornithine decarboxylase in Saccharomyces cerevisiae and Saccharomyces uvarum by using alpha-[5-14C]difluoromethylornithine

Biochimica Et Biophysica Acta
H Pösö, A E Pegg

Abstract

Ornithine decarboxylase (EC 4.1.1.17) activity was about 3-times higher in Saccharomyces uvarum than in Saccharomyces cerevisiae in the middle of logarithmic growth. The enzyme from both sources was inactivated by alpha-difluoromethylornithine. When the binding of [5-14 C]difluoromethylornithine to yeast ornithine decarboxylase was studied it was shown that S. uvarum extracts contained about 40 ng of active ornithine decarboxylase per mg of cellular protein, and that of S. cerevisiae 10-12 ng of active enzyme. It appeared that S. uvarum ornithine decarboxylase could be highly purified by affinity chromatography, but S. cerevisiae enzyme did not bind to the same column. The purified preparation from S. uvarum had an Mr of 73 000 and a 100-fold purification (purified by conventional methods) of ornithine decarboxylase from S. cerevisiae had an Mr of 69 000 on a gel filtration column. When the purified S. ovarum ornithine decarboxylase was labelled with difluoromethylornithine, it co-eluted with native enzyme on a gel filtration column and it ran as a single band on polyacrylamide gel electrophoresis under denaturing conditions at a position corresponding to an Mr of 72 000, indicating that the active enzyme is a monomer. The loss...Continue Reading

References

Jun 9, 1978·Biochimica Et Biophysica Acta·H PösöJ Jänne
Jan 1, 1978·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·A Kallio
Oct 1, 1975·The Biochemical Journal·H PösöJ Jänne
Jun 1, 1981·Biochemical and Biophysical Research Communications·M L PritchardA E Pegg
Feb 11, 1982·Biochemical and Biophysical Research Communications·H SchwaigerW Tanner
Jan 15, 1981·Biochimica Et Biophysica Acta·J L MitchellJ M Wilson
Dec 9, 2003·Applied and Environmental Microbiology·P H M LeungW C Yam
Jan 1, 1982·Applied Biochemistry and Biotechnology·L Häggström, S O Enfors

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