Measurement of the Spatial Distribution of S1P in Small Quantities of Tissues: Development and Application of a Highly Sensitive LC-MS/MS Method Combined with Laser Microdissection

Mass Spectrometry
Jiao WangJunken Aoki

Abstract

Sphingosine-1-phosphate (S1P) acts as an extracellular signaling molecule with diverse biological functions. Tissues appear to have an S1P gradient, which is functionally relevant in the biological significance of S1P, although its existence has not been measured directly. Here, we report a highly sensitive method to determine the distribution of S1P, using a column-switching LC-MS/MS system combined with laser microdissection (LMD). Column switching using narrow core Capcell Pak C18 analytical and trap columns with 0.3 mm inner diameter improved the performance of the LC-MS/MS system. The calibration curve of S1P showed good linearity (r>0.999) over the range of 0.05-10 nM (1-200 fmol/injection). The accuracy of the method was confirmed by measuring S1P-spiked laser microdissected mice tissue sections. To evaluate our S1P analytical method, we quantified S1P extracted from micro-dissected mouse brain and spleen. These results show that this method can measure low S1P concentrations and determine S1P distribution in tissue microenvironments.

References

Oct 28, 2006·Toxicologic Pathology·Mark F Cesta
Jul 12, 2007·Journal of Neurochemistry·Nicolas BlondeauChristian Waeber
Oct 13, 2007·Journal of Neurochemistry·Koichi SatoFumikazu Okajima
May 20, 2008·Biochimica Et Biophysica Acta·Lauren BryanSheldon Milstien
Mar 30, 2011·Nature Chemical Biology·Stuart M CahalanHugh Rosen
Oct 18, 2011·Trends in Cell Biology·Michael MaceykaSarah Spiegel
Dec 14, 2011·Annual Review of Immunology·Jason G Cyster, Susan R Schwab
Jan 25, 2014·Journal of Lipid Research·Victoria A Blaho, Timothy Hla
Mar 29, 2014·The Journal of Clinical Investigation·Mari KonoRichard L Proia
Jul 30, 2014·Analytical Chemistry·E Ellen JonesRichard R Drake
May 28, 2015·Journal of Lipid Research·Monika KsiążekMarcin Baranowski
Oct 27, 2015·Nature Immunology·Willy D Ramos-PerezSusan R Schwab
Jan 1, 2014·Mass Spectrometry·Daisuke SaigusaJunken Aoki
Oct 29, 2017·Nature Communications·Mari KonoRichard L Proia

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Citations

Aug 19, 2021·Inflammation and Regeneration·Ryota Hokari, Akira Tomioka
Nov 24, 2021·Annual Review of Pathology·Kuniyuki KanoTimothy Hla

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Methods Mentioned

BETA
dissection

Software Mentioned

Xcalibur

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