Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)

PloS One
Lenka A VodstrcilJane S Hocking

Abstract

Azithromycin is recommended for the treatment of uncomplicated urogenital chlamydia infection although the standard 1gram dose sometimes fails to eradicate the infection (treatment failure). One hypothesis proposed for treatment failure has been insufficient levels of the antibiotic at the site of infection. We developed an assay using liquid chromatography and tandem mass spectrometry (LC-MS/MS) to measure azithromycin concentration in high-vaginal swabs and monitor how concentration changes over time following routine azithromycin treatment. Azithromycin concentrations were measured in two groups of women either within the first 24h of taking a 1g dose (N = 11) or over 9 days (N = 10). Azithromycin concentrations were normalised to an internal standard (leucine enkephalin), and the bulk lipid species phosphatidylcholine [PC(34:1)], using an Agilent 6490 triple quadrupole instrument in positive ionisation mode. The abundances of azithromycin, PC(34:1), and leu-enkephalin were determined by multiple reaction monitoring and absolute levels of azithromycin estimated using standard curves prepared on vaginal specimens. Vaginal azithromycin concentrations of women were rapidly obtained after 5h post-treatment (mean concentration = ...Continue Reading

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Citations

Nov 21, 2018·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Jingchen TianXingang Li
Jan 17, 2019·The Journal of Antimicrobial Chemotherapy·Fabian Yuh Shiong KongJane S Hocking
Mar 30, 2017·PloS One·Fabian Y S KongJane S Hocking

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Agilent MassHunter
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