Measurement of tissue cholecystokinin (CCK) concentrations by bioassay and specific radioimmunoassay: characterization of the bioactivity of CCK-58 before and after tryptic cleavage

European Journal of Clinical Investigation
M HöckerU R Fölsch

Abstract

Cholecystokinin (CCK) was measured in an extract of porcine brain by a bioassay system based on the enzyme release from isolated pancreatic acini and a specific radioimmunoassay. The tissue extract represented a crude peptide preparation of porcine brain that contained several molecular forms of CCK, most of them of high molecular weight including CCK-58. The different molecular forms of CCK in the porcine brain extract were isolated by high performance liquid chromatography (HPLC). Determination of CCK-like bioactivity and immunoreactivity after HPLC was performed in parallel by bioassay and radioimmunoassay, respectively CCK-58 was the most abundant molecular form in the porcine brain extract, followed by CCK-33, and CCK-8. Both assay systems measured similar relative concentrations of all different molecular forms of CCK. To elucidate the biological potency of CCK-58 before and after tryptic cleavage, a CCK-58-enriched fraction was prepared by HPLC. Part of this material was quantitatively cleaved by trypsin resulting in the formation of small molecular forms of CCK. Bioactivity and immunoreactivity of equal amounts of cleaved and uncleaved material (= CCK-58) were determined by bioassay and radioimmunoassay in parallel. Cle...Continue Reading

References

Sep 3, 1985·Journal of Immunological Methods·P Cantor, J F Rehfeld
Mar 1, 1985·The Biochemical Journal·R BruzzoneE R Trimble
Apr 1, 1988·Scandinavian Journal of Gastroenterology·A SchafmayerH Köhler
Jul 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·J B Jansen, C B Lamers

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Citations

Mar 23, 1994·Annals of the New York Academy of Sciences·J R ReeveC Evans

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