Measurement of tissue volume during non-steady state high-intensity muscle contraction

The American Journal of Physiology
D S WardP D Watson

Abstract

To investigate the pressures driving water into stimulated muscle, water distribution during and after muscle stimulation was studied in isolated cat muscles perfused by recirculating diluted blood. 51Cr-labeled EDTA (51Cr-EDTA) and Evans blue-labeled albumin were used to determine extracellular volume and plasma volume (PV), respectively. Change in tissue volume was calculated as -PV. Interstitial volume (IFV) was determined from the ratio of interstitial solute (51Cr-EDTA and sodium) mass and interstitial concentration. Interstitial mass was determined by mass balance, and interstitial concentration was determined from solute flux and Fick's Law. One group was stimulated at 4 Hz for 2 min, and a second was stimulated by 80-Hz trains (1 train/s, 0.1 s duration). Four Hertz stimulation increased total tissue volume by approximately 3 ml/100 g and decreased IFV by 1 ml/100 g. Train stimulation increased total tissue volume by 6 ml/100 g and decreased IFV by 4. These data indicate that water moves into cells faster than the simultaneous transcapillary flow, suggesting that intracellular osmoles provide the primary driving pressure in stimulation-induced swelling.

Citations

Oct 4, 2000·Physiological Reviews·O M Sejersted, G Sjøgaard
Feb 13, 2001·Journal of Applied Physiology·B M PriorR A Meyer
Oct 17, 2002·Journal of Applied Physiology·Anders T Nygren, Lennart Kaijser
May 8, 2020·Physiological Reports·Steffen WillwacherGrischa Bratke
Sep 29, 2019·The Journal of Experimental Biology·David A SlebodaThomas J Roberts
Apr 28, 2021·Physiological Reports·Mirko MandićEric Rullman
Jan 18, 2022·European Journal of Applied Physiology·Michael I Lindinger

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