Measurement of unimolecular decay in peptides of masses greater than 1200 units by a reflecting time-of-flight mass spectrometer

Rapid Communications in Mass Spectrometry : RCM
X J TangJ B Westmore

Abstract

Daughter ions from decomposition of [M + H]+ parent ions have been observed in a time-of-flight mass spectrometer fitted with an ion mirror. Unit mass resolution is obtained for parent ions of masses up to several thousand u when the mirror voltage is set at a value determined by the usual velocity-focusing criterion for parent ions. Under this condition the daughter-ion resolution in the low-mass range suffers. Considerable improvement is obtained when the daughter-ion spectrum is examined in several segments, with the mirror voltage optimized for each segment. The daughter-ion spectrum from decay of metastable [M + H]+ parent ions in Substance P is measured using this technique. Reasons are suggested for differences between the spectrum obtained and a spectrum for the same compound recently obtained using a tandem double-focusing instrument.

References

Aug 1, 1988·Biomedical & Environmental Mass Spectrometry·J A HillK Biemann
Sep 1, 1988·Rapid Communications in Mass Spectrometry : RCM·S J Gaskell, M H Reilly
Jul 1, 1989·Rapid Communications in Mass Spectrometry : RCM·G C Thorne, S J Gaskell
Jul 1, 1988·Rapid Communications in Mass Spectrometry : RCM·S J GaskellC J Porter
Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·K Biemann
Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·S J GaskellM H Reilly
Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·A H Lawrence, A A Nanji
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F HuntC R Hauer
Nov 1, 1984·Biomedical Mass Spectrometry·P Roepstorff, J Fohlman

❮ Previous
Next ❯

Citations

Mar 1, 1996·Journal of the American Society for Mass Spectrometry·R S BrownJ J Lennon
Mar 30, 2004·Journal of the American Society for Mass Spectrometry·Andrew J Hoteling, Kevin G Owens
May 1, 1994·Rapid Communications in Mass Spectrometry : RCM·S T FountainD M Lubman
Feb 1, 2006·Rapid Communications in Mass Spectrometry : RCM·David TouboulOlivier Laprévote
Jul 8, 2015·Journal of Mass Spectrometry : JMS·Boone M PrenticeRichard M Caprioli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.