Measurement of urea and creatinine in saliva of dogs: a pilot study

BMC Veterinary Research
Asta TvarijonaviciuteSilvia Martinez-Subiela

Abstract

Urea and creatinine in saliva have been reported to be possible markers of chronic kidney disease (CKD) in humans. The aim of this study was to assess if urea and creatinine could be measured in canine saliva, and to evaluate their possible changes in situations of CKD. The spectrophotometric assays for urea and creatinine measurements in saliva of dogs showed intra- and inter-assay imprecision lower than 12% and coefficients of correlation close to 1 in linearity under dilution tests. Healthy dogs showed median salivary concentrations of urea of 39.6 mg/dL and creatinine of 0.30 mg/dL, whereas dogs with CKD showed median salivary urea of 270.1 mg/dL and creatinine of 1.86 mg/dL. Positive high correlations were found between saliva and serum activities of the two analytes (urea, r = 0.909; P < 0.001; creatinine, r = 0.819; P < 0.001). Urea and creatinine concentrations can be measured in canine saliva with commercially available spectrophotometric assays. Both analytes showed higher values in saliva of dogs with CKD compared with healthy dogs and their values were highly correlated with those in serum in our study conditions.

References

Dec 1, 1992·Veterinary Parasitology·C G NietoE Redondo
Dec 5, 2003·Veterinary Clinical Pathology·J P BraunA D J Watson
Apr 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·María Dolores ParraJosé Joaquín Cerón
Feb 26, 2008·Archives of Oral Biology·I TomásP Diz
Nov 26, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·Estela M L CardosoLiliana N Contreras
Mar 20, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Esti DavidovichDoron J Aframian
Aug 14, 2010·Domestic Animal Endocrinology·A Bennett, V Hayssen
Mar 9, 2011·Clinical Chemistry·Tina PfaffeChamindie Punyadeera
Sep 20, 2011·Journal of Nephrology·Maria E ZúñigaJavier A Cieza
Nov 6, 2012·Clinical Biochemistry·Can-hui PengPing Xiao
May 18, 2013·The Veterinary Journal·Xavier RouraEric Zini
May 13, 2014·International Journal of Nephrology·Ramesh VenkatapathyKarthikshree V Prashad
Jan 18, 2016·BMC Nephrology·Taye Jemilat LasisiBabatunde Lawal Salako
Feb 27, 2016·Veterinary Clinical Pathology·Jessica A Hokamp, Mary B Nabity
Mar 22, 2016·Saudi Journal of Kidney Diseases and Transplantation : an Official Publication of the Saudi Center for Organ Transplantation, Saudi Arabia·Naresh YajamanamP V L N Srinivasa Rao
Jun 11, 2017·BMC Veterinary Research·Asta TvarijonaviciuteJosé J Cerón

❮ Previous
Next ❯

Citations

Jun 7, 2019·BMC Veterinary Research·María Dolores Contreras-AguilarJose Joaquín Cerón
Aug 12, 2019·Veterinary Clinical Pathology·Lucia SanchiniTim L Williams

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

Graph Pad Prism
Graph Padsoftware

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Vétérinaire
Asta TvarijonaviciuteJosé J Cerón
Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Damián EscribanoJosé J Cerón
Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
María FuentesJosé J Cerón
The Veterinary Record
Asta TvarijonaviciuteJuan Diego Garcia-Martinez
© 2022 Meta ULC. All rights reserved