Measurement of water transport during freezing in cell suspensions using a differential scanning calorimeter

Cryobiology
R V DevireddyJ C Bischof

Abstract

A new technique using a differential scanning calorimeter (DSC) was developed to obtain dynamic and quantitative water transport data in cell suspensions during freezing. The model system investigated was a nonattached spherical lymphocyte (Epstein-Barr virus transformed, EBVT) human cell line. Data from the technique show that the initial heat release of a prenucleated sample containing osmotically active cells in media is greater than the final heat release of an identical sample of osmotically inactive or lysed cells in media. The total integrated magnitude of this difference, Deltaqdsc, was found to be proportional to the cytocrit and hence also to the supercooled water volume in the sample. Further, the normalized fractional integrated heat release difference as a function of temperature, Deltaq(T)dsc/Deltaqdsc, was shown to correlate with the amount of supercooled cellular water which had exosmosed from the cell as a function of subzero temperature at constant cooling rates of 5, 10, and 20 degrees C/min. Several important limitations of the technique are (1) that it requires a priori knowledge of geometric parameters such as the surface area, initial volume, and osmotically inactive cell volume and (2) that the technique...Continue Reading

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Citations

Dec 21, 2000·ILAR Journal·D Gao, J K Critser
Jun 19, 2009·Biology of Reproduction·Mie HagiwaraJohn C Bischof
Jan 26, 1999·Annals of the New York Academy of Sciences·P R BarrattJ C Bischof
Mar 1, 2006·Journal of Biomechanical Engineering·Ram V DevireddyJeffrey M Gimble
Dec 24, 2008·Journal of Biomechanical Engineering·Bumsoo HanJun K Jung
Jul 31, 2009·Journal of Biomechanical Engineering·Alptekin AksanJohn C Bischof
Nov 10, 2001·Annual Review of Biomedical Engineering·J C Bischof
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·G LiR V Devireddy
Jun 12, 2014·Biopreservation and Biobanking·Allison HubelAmy P N Skubitz
Jun 24, 2005·Journal of Biomechanical Engineering·Sreedhar Thirumala, Ram V Devireddy
Aug 12, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Huiping Yang, Terrence R Tiersch
Apr 29, 2005·Journal of Microscopy·S Yuan, K R Diller
Sep 13, 2005·Biotechnology and Bioengineering·Sreedhar ThirumalaRam V Devireddy
Mar 3, 2004·Molecular Reproduction and Development·R V DevireddyS P Leibo
Feb 24, 2009·Biochimica Et Biophysica Acta·Saravana K BalasubramanianJohn C Bischof
Apr 7, 2012·Molecular Membrane Biology·Harriëtte OldenhofHarald Sieme
Jul 27, 2012·Molecular Membrane Biology·Maryam AkhoondiWillem F Wolkers
Oct 16, 2012·Theriogenology·J D BensonJ K Critser
Dec 4, 2010·Biochimica Et Biophysica Acta·Maryam AkhoondiWillem F Wolkers
Jan 25, 2002·Biophysical Journal·Farshid GuilakH Ping Ting-Beall

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