Measurements of mean initial velocities of analyte and matrix ions in infrared matrix-assisted laser desorption ionization mass spectrometry

Journal of the American Society for Mass Spectrometry
S BerkenkampKlaus Dreisewerd

Abstract

The mean initial velocities of analyte ions ranging in molecular weight from 1000 Da to 150 kDa and desorbed with a pulsed Er:YAG laser from various solid-state and liquid IR MALDI matrices were measured along with those of the matrix ions. Experiments with UV MALDI were performed for comparison in addition for a 2,5-dihydroxybenzoic acid preparation. Two different measurement principles were employed, (1) a delayed extraction method, relying on the initial velocity-dependent increase of flight times with delay time between laser and HV ion extraction pulse, and (2) a field-free drift method in which the first region of a two-stage ion source was varied in length and the flight times compared. The two methods yielded somewhat different values for the mean initial ion velocities. Based on a detailed discussion of the measurement principles it is suggested that the actual initial velocities of IR MALDI ions lie between the limits set by the two methods. The influences of the analyte-to-matrix ratio, laser fluence, and laser wavelength on the initial ion velocities were also investigated. Significant differences between the desorption mechanisms for liquid and solid-state matrices were observed.

References

Nov 1, 1992·Rapid Communications in Mass Spectrometry : RCM·J ZhouA Verentchikov
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S BerkenkampF Hillenkamp
Jul 1, 1997·Journal of Protein Chemistry·E J TakachS A Martin
Sep 15, 1987·Physical Review. B, Condensed Matter·I NoorBatchaY Zeiri

❮ Previous
Next ❯

Citations

Aug 30, 2008·Analytical and Bioanalytical Chemistry·Sasa M MiladinovicCharles L Wilkins
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Yong Jin BaeMyung Soo Kim
May 18, 2004·Journal of the American Society for Mass Spectrometry·Arne LeisnerKlaus Dreisewerd
Oct 4, 2005·Journal of the American Society for Mass Spectrometry·Gregg A CzerwieniecEric E Gard
Sep 27, 2005·Journal of the American Society for Mass Spectrometry·Arwah J JaberCharles L Wilkins
Jan 6, 2007·Journal of the American Society for Mass Spectrometry·Jennifer M CampbellAlfred L Yergey
Sep 10, 2002·Journal of the American Society for Mass Spectrometry·Christoph MenzelFranz Hillenkamp
Aug 26, 2014·Journal of the American Society for Mass Spectrometry·Vitaliy ChagovetsRenato Zenobi
Sep 1, 2007·International Journal of Mass Spectrometry·Serguei Ilchenko, Robert J Cotter
Jul 24, 2003·Rapid Communications in Mass Spectrometry : RCM·J-F GreischE De Pauw
Sep 2, 2011·Chemistry, an Asian Journal·Chi-Wei LiangChi-Kung Ni
Mar 31, 2004·Rapid Communications in Mass Spectrometry : RCM·Kevin Turney, W W Harrison
May 18, 2016·Physical Chemistry Chemical Physics : PCCP·E Alonso, R Zenobi
Jul 3, 2016·Analytical and Bioanalytical Chemistry·José VillatoroHans-Gerd Löhmannsröben
Dec 15, 2016·Journal of the American Society for Mass Spectrometry·Avinash A PatilWen-Ping Peng
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Dec 9, 2017·Journal of the American Society for Mass Spectrometry·Brian J MalysKevin G Owens
Feb 13, 2003·Chemical Reviews·Leonid V ZhigileiMichael I Zeifman
Feb 13, 2003·Chemical Reviews·Klaus Dreisewerd
Feb 13, 2003·Chemical Reviews·Michael Karas, Ralf Krüger
Mar 26, 2013·The Journal of Physical Chemistry. B·Chi Wei LiangChi Kung Ni
Jul 6, 2014·The Journal of Physical Chemistry. a·Bryana L Henderson, Murthy S Gudipati
Apr 3, 2020·Journal of the American Society for Mass Spectrometry·Hou-Yu LinChi-Kung Ni
Jul 15, 2005·Analytical Chemistry·Ryan J WenzelRenato Zenobi

❮ Previous
Next ❯

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.