PMID: 16662666Nov 1, 1982

Measurements of membrane potentials in plant mitochondria with the safranine method

Plant Physiology
A L Moore, W D Bonner

Abstract

The positively charged dye, safranine, has been used as an indicator of membrane potentials in mung bean (Phaseolus aureus) and Voodoo lily (Sauromatum guttatum) mitochondria under a variety of metabolic conditions. The spectral response of safranine has been calibrated with respect to a K(+) diffusion potential and was found to be linearly related to the developed potential within the range of 50 to 160 millivolts. Both respiration and ATP hydrolysis gave rise to a membrane potential of approximately 135 millivolts. Respiratory inhibitors such as cyanide and antimycin depolarized the potential, whereas rotenone has little effect. No potentials were developed during NADH supported cyanide insensitive respiration. It is concluded that safranine may be a useful spectrophotometric probe of the mitochondrial membrane potential.

References

Jan 1, 1977·Annual Review of Biochemistry·E C Slater
Mar 1, 1978·Archives of Biochemistry and Biophysics·A L MooreP R Rich
May 2, 1973·European Journal of Biochemistry·R ColnnaG F Azzone
Apr 15, 1980·Archives of Biochemistry and Biophysics·A Zanotti, G F Azzone

Citations

Feb 23, 1994·Biochimica Et Biophysica Acta·A Ballarin-DentiH Kuroda
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T E Elthon, L McIntosh
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D M Rhoads, L McIntosh
May 31, 2008·Plant & Cell Physiology·M N LausD Pastore
Dec 1, 1988·Plant Physiology·B K Ishida, J M Palmer
Jan 8, 2008·Journal of Plant Physiology·Elisa PetrussaA Vianello
Nov 5, 2015·Journal of Bioenergetics and Biomembranes·Alfredo Cabrera-OreficeSalvador Uribe-Carvajal
May 10, 2007·The FEBS Journal·Gianluca PaventiS Passarella
Jul 12, 2011·Cell Biochemistry and Function·Vitali T CheshchevikIlya B Zavodnik
Jun 30, 2006·The International Journal of Biochemistry & Cell Biology·P JezekMartin Jabůrek
Aug 23, 1991·Biochimica Et Biophysica Acta·A L Moore, J N Siedow
May 14, 2014·International Journal of Molecular Sciences·D TronoD Pastore
Jan 22, 2002·FEBS Letters·C AffourtitAnthony L Moore
Sep 10, 1999·The Journal of Biological Chemistry·D PastoreS Passarella
May 15, 2001·The Journal of Biological Chemistry·C AffourtitA L Moore
Feb 24, 2017·Physiology International·N G KrylovaIlya B Zavodnik
Sep 3, 2004·The Journal of Biological Chemistry·Frank KrauseNorbert A Dencher
Dec 12, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Marten SziborAnthony L Moore
Feb 20, 2021·Biochimie·Vitali T CheshchevikIlya B Zavodnik

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