PMID: 9421971Jan 9, 1998Paper

Measurements of the protective effect of topically applied sunscreens using in vitro three-dimensional dermal and skin equivalents

Photochemistry and Photobiology
C AugustinOdile Damour

Abstract

For preventing or minimizing acute and chronic skin damage caused by UV radiation, the use of sunscreens is probably the most important measure. To screen the protective efficacy of new sunscreen molecules or formulations against UV rays, we evaluated as in vitro testing methods the use of two three-dimensional models, a dermal equivalent (DE) and a skin equivalent (SE). The DE is composed of a porous collagen-glycosaminoglycans-chitosan matrix populated by normal human fibroblasts. The SE is comprised of a fully differentiated epidermis realized by seeding keratinocytes onto the DE. In this study, we demonstrated that the DE and SE models react to the deleterious effects of UVA and UVB. Then, we extended our research to the evaluation of their usefulness for photoprotection trials. Sunscreen agents (Eusolex 8020 and 6300) and commercially available sunscreens (chemical and physical filter formulations) that protect the skin against either UVA or UBV were evaluated. The tested products were applied (n = 6) topically (10 microL) and incubated for 30 min prior to irradiation over a range of UVA (0-50 J/cm2) or UVB (0-5 J/cm2). The photoprotection provided by the tested sunscreen molecules and formulations was evaluated by measure...Continue Reading

References

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Citations

Jun 1, 2000·Medical & Biological Engineering & Computing·G VaissiereO Damour
Jul 7, 2005·Tissue Engineering·Annie F BlackOdile Damour
Feb 1, 1997·Photodermatology, Photoimmunology & Photomedicine·C AugustinO Damour
Feb 10, 2016·Journal of Tissue Engineering and Regenerative Medicine·G ImparatoP A Netti
Aug 25, 2009·Biomaterials·Beste KinikogluOdile Damour
May 4, 2013·Pharmaceutical Development and Technology·Sachie YoshinoKenji Sugibayashi
Jun 15, 1999·Medical & Biological Engineering & Computing·O DamourA F Black
Mar 8, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Gabriele VielhaberJean Krutmann
Jul 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·C AuxenfansO Damour
Aug 20, 2003·Photodermatology, Photoimmunology & Photomedicine·R GilliesN Kollias
Jan 29, 2000·The Journal of Investigative Dermatology·F Duplan-PerratF Braye

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