Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay

The Journal of General and Applied Microbiology
Takashi SaitoYasuhiko Sekine

Abstract

Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using "GFP hop-on assay" in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Ohtsubo, E Ohtsubo
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Sekine, E Ohtsubo
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S IidaW Arber
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Nov 25, 1980·Journal of Molecular Biology·D J GalasJ H Miller
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Dec 31, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y SekineE Ohtsubo
Mar 29, 2001·Journal of Bacteriology·Y ShigaE Ohtsubo
Oct 26, 2002·Journal of Bacteriology·Shinya OhtaEiichi Ohtsubo
Feb 20, 2007·Biochemical and Biophysical Research Communications·Kiwamu TakahashiHirofumi Yoshikawa

❮ Previous
Next ❯

Citations

Dec 7, 2013·Biology Letters·Ana SousaIsabel Gordo
Mar 9, 2021·ELife·Anna Nagy-StaronCalin C Guet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.