Measures of kidney function by minimally invasive techniques correlate with histological glomerular damage in SCID mice with adriamycin-induced nephropathy

Scientific Reports
Lauren ScarfeBettina Wilm

Abstract

Maximising the use of preclinical murine models of progressive kidney disease as test beds for therapies ideally requires kidney function to be measured repeatedly in a safe, minimally invasive manner. To date, most studies of murine nephropathy depend on unreliable markers of renal physiological function, exemplified by measuring blood levels of creatinine and urea, and on various end points necessitating sacrifice of experimental animals to assess histological damage, thus counteracting the principles of Replacement, Refinement and Reduction. Here, we applied two novel minimally invasive techniques to measure kidney function in SCID mice with adriamycin-induced nephropathy. We employed i) a transcutaneous device that measures the half-life of intravenously administered FITC-sinistrin, a molecule cleared by glomerular filtration; and ii) multispectral optoacoustic tomography, a photoacoustic imaging device that directly visualises the clearance of the near infrared dye, IRDye 800CW carboxylate. Measurements with either technique showed a significant impairment of renal function in experimental animals versus controls, with significant correlations with the proportion of scarred glomeruli five weeks after induction of injury. T...Continue Reading

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Citations

Apr 5, 2017·Stem Cells Translational Medicine·Ilaria SanteramoNorbert Gretz
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Sep 2, 2020·Medicina·Hasanain AlaridheeCordula M Stover
Apr 20, 2018·Oxidative Medicine and Cellular Longevity·Lei GaoChristopher W K Lai
Mar 30, 2021·Clinical Kidney Journal·Stephen J McWilliamLouise Oni
May 29, 2021·Scientific Reports·Xiaoyuan YangSarah Y Yuan

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Methods Mentioned

BETA
urine collections
equilibrium dialysis
urine collection
ELISA

Software Mentioned

ImageJ
Matlab
Fiji

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