Measuring Activity of Native Plant Sirtuins - The Wheat Mitochondrial Model

Frontiers in Plant Science
Mario SoccioDonato Pastore

Abstract

Sirtuins are NAD+-dependent deacetylase enzymes that have gained considerable interest in mammals for their recognized importance in gene silencing and expression and in cell metabolism. Conversely, knowledge about plant sirtuins remains limited, although a sirtuin-mediated regulation of mitochondrial energy metabolism has been recently reported in Arabidopsis. However, so far, no information is available about direct measurement of intracellular plant sirtuin activity, i.e., in cell extracts and/or subcellular organelles. In this study, a novel approach was proposed for reliable evaluation of native sirtuin activity in plant samples, based on (i) an adequate combinatory application of enzymatic assays very different for chemical basis and rationale and (ii) a comparative measurement of activity of a recombinant sirtuin isoform. In particular, two sirtuin assays were applied, based on bioluminescence emission and Homogeneous Time-Resolved Fluorescence (HTRF®) technology, and the human SIRT1 isoform (hSIRT1) was used for comparison. For the first time in plants, this new approach allowed measuring directly a high and nicotinamide-sensitive sirtuin activity in highly purified mitochondrial fraction obtained from durum wheat (WM)....Continue Reading

References

Sep 26, 2002·The Journal of Biological Chemistry·Kevin J BittermanDavid A Sinclair
Aug 27, 2003·Nature·Konrad T HowitzDavid A Sinclair
Oct 3, 2003·The Journal of Biological Chemistry·Michael D JacksonJohn M Denu
Feb 3, 2005·The Journal of Biological Chemistry·Matt KaeberleinBrian K Kennedy
Mar 8, 2005·The Journal of Biological Chemistry·Margie T BorraJohn M Denu
Apr 11, 2006·Mechanisms of Ageing and Development·Vincent C J de BoerJaap Keijer
Oct 27, 2006·Biochemical and Biophysical Research Communications·Adel BakhtiarovaDaniel M Kemp
Apr 24, 2007·The Biochemical Journal·Shaday Michan, David Sinclair
Mar 28, 2008·Journal of Medicinal Chemistry·Douglas S AuldJames Inglese
May 31, 2008·Plant & Cell Physiology·Maura Nicoletta LausDonato Pastore
Aug 21, 2008·Journal of Integrative Plant Biology·Courtney Hollender, Zhongchi Liu
Nov 11, 2008·Molecular Cell·Joshua C BlackMichael Carey
Jun 10, 2009·FEBS Letters·Pil Joong ChungJu-Kon Kim
Sep 22, 2009·Biochimica Et Biophysica Acta·Melanie Gertz, Clemens Steegborn
Jan 12, 2010·The Journal of Biological Chemistry·Michelle PacholecKay Ahn
Jan 19, 2010·Annual Review of Pathology·Marcia C Haigis, David A Sinclair
May 11, 2010·Archives of Biochemistry and Biophysics·Sangwoon ChungIrfan Rahman
Jun 11, 2011·Molecular Cell·Eric L Bell, Leonard Guarente
Mar 30, 2012·Current Medicinal Chemistry·S Sanchez-FidalgoC Alarcon de la Lastra
Dec 26, 2012·Plant Science : an International Journal of Experimental Plant Biology·Daniela TronoDonato Pastore
Mar 9, 2013·Science·Basil P HubbardDavid A Sinclair
Mar 26, 2013·Aging·Mahadevan LakshminarasimhanClemens Steegborn
Jan 21, 2014·Trends in Pharmacological Sciences·Basil P Hubbard, David A Sinclair
May 14, 2014·International Journal of Molecular Sciences·Daniela TronoDonato Pastore
Jan 23, 2015·Frontiers in Plant Science·Linmao ZhaoKeqiang Wu
Oct 22, 2016·Cell Cycle·Wenlong Bai, Xiaohong Zhang
May 12, 2017·Analytical Chemistry·Huateng ZhangMinyong Li
Oct 17, 2017·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Dorota RogackaAgnieszka Piwkowska
Oct 25, 2017·Molecular Systems Biology·Markus HartlIris Finkemeier

❮ Previous
Next ❯

Citations

May 5, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Karin KrupinskaMichela Zottini
May 18, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Valentina Lukinović, Kyle K Biggar

❮ Previous
Next ❯

Datasets Mentioned

BETA
ABA95936

Methods Mentioned

BETA
acetylation
Fluorescence
Assay
FRET

Software Mentioned

EnsemblPlants
Vector NTI Suite
blastp
Predotar
Ensembl Plants
TargetP
iPSORT
MitoProt
Cisbio

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.