Apr 10, 2020

DivIVA is essential in Deinococcus radiodurans and its C terminal domain regulates new septum orientation during cell division

BioRxiv : the Preprint Server for Biology
Reema ChaudharyH S Misra


FtsZ assembly at mid cell position in rod shaped bacteria is regulated by gradient of MinCDE complex across the poles. In round shaped bacteria, which lack predefined poles and the next plane of cell division is perpendicular to previous plane, the determination of site for FtsZ assembly is intriguing. Deinococcus radiodurans a coccus shaped bacterium, is characterized for its extraordinary resistance to DNA damage. Here we report that DivIVA a putative component of Min system in this bacterium (drDivIVA) interacts with cognate cell division and genome segregation proteins. The deletion of full length drDivIVA was found to be indispensable while its C-terminal deletion ({Delta}divIVAC) was dispensable but produced distinguishable phenotypes like slow growth, altered plane for new septum formation and angular septum. Both wild type and mutant showed FtsZ foci formation and their gamma radiation responses were nearly identical. But unlike in wild type, the FtsZ localization in mutant cells was found to be away from orthogonal axis with respect to plane of previous septum. Notably, DivIVA-RFP localizes to membrane during cell division and then perpendicular to previous plane of cell division. In trans expression of drDivIVA in {De...Continue Reading

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Mentioned in this Paper

Crystal - Body Material
Cyclophilin A
Enzymes, antithrombotic
FUS gene
Diffusion Weighted Imaging
Protein Function
Crystallography, X-Ray
PPIA gene

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