PMID: 15361726Sep 14, 2004Paper

Measuring antibody responses to a live attenuated influenza vaccine in children

The Pediatric Infectious Disease Journal
Min-Shi LeePaul M Mendelman

Abstract

Hemagglutination inhibition (HAI) assay is the standard method for evaluating inactivated influenza vaccines, but no standard assay has been established for evaluating live attenuated influenza vaccines (LAIV). LAIV containing A/Beijing/262/95(H1N1) induced low serum HAI antibody responses to the antigenic variant, A/New Caledonia/20/99(H1N1) in a serologic study but provided protection against the A/New Caledonia-like viruses in a community study. Neutralization and HAI assays were compared by measuring H1N1 cross-reactive antibody responses to the LAIV in children. Sera were collected from 50 children 1-8 years of age before vaccination and 4-6 weeks after each dose of the LAIV. Antibody titers to the 3 vaccine viruses were measured by the HAI assay, whereas antibody titers against the H1N1 vaccine virus (A/Beijing/262/95) and 2 H1N1 antigenic variants (A/Shenzhen/227/95 and A/New Caledonia/20/99) were measured by the HAI and neutralization assays. Initially seronegative participants were more likely to develop HAI seroconversion responses to the 3 vaccine viruses than the baseline seropositive participants (77% versus 14% for H1N1, 100% versus 20% for H3N2, 100% versus 19% for B, P < 0.01, Fisher's exact test). For the H1N1 ...Continue Reading

References

May 23, 2000·The Pediatric Infectious Disease Journal·R B Belshe, W C Gruber
Jan 27, 2004·The Pediatric Infectious Disease Journal·Stan L Block

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Citations

Oct 11, 2011·The Pediatric Infectious Disease Journal·Steven BlackRino Rappuoli
Aug 21, 2008·PloS One·Sanae SasakiHarry B Greenberg
Oct 22, 2004·Emerging Infectious Diseases·Min-Shi Lee, Jack Si-En Chen
Dec 20, 2015·Future Microbiology·Marios KoutsakosKatherine Kedzierska
Apr 16, 2015·Human Vaccines & Immunotherapeutics·Nagendra R Hegde
Nov 27, 2014·The Journal of Infectious Diseases·Kristin G-I MohnRebecca J Cox
Sep 25, 2012·Vaccine·Christopher S AmbroseRaburn M Mallory
Aug 26, 2016·MMWR. Recommendations and Reports : Morbidity and Mortality Weekly Report. Recommendations and Reports·Lisa A GrohskopfJoseph S Bresee
May 2, 2008·Clinical and Vaccine Immunology : CVI·Bruce D ForrestWilliam C Gruber
Nov 26, 2010·Expert Review of Vaccines·Zichria Zakay-Rones
Jul 3, 2020·Human Vaccines & Immunotherapeutics·Marina S BoukhvalovaJorge C G Blanco
Nov 16, 2012·Expert Review of Vaccines·Seth L TobackJudith Falloon

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