Measuring Cellular Ion Transport by Magnetoencephalography

ACS Omega
Sudhir Kumar SharmaRamesh Jagannathan

Abstract

The cellular-level process of ion transport is known to generate a magnetic field. A noninvasive magnetoencephalography (MEG) technique was used to measure the magnetic field emanating from HeLa, HEK293, and H9c2(2-1) rat cardiac cells. The addition of a nonlethal dose of ionomycin to HeLa and capsaicin to TRPV1-expressing HEK293 cells resulted in a sudden change in the magnetic field signal consistent with Ca2+ influx, which was also observed by confocal fluorescence microscopy under the same conditions. In contrast, addition of capsaicin to TRPV1-expressing HEK293 cells containing an optimum amount of a TRPV1 antagonist (ruthenium red), resulted in no detectable magnetic or fluorescent signals. These signals confirmed that the measured MEG signals are due to cellular ion transport through the cell membrane. In general, there is evidence that ion channel/transporter activation and ionic flux are linked to cancer. Therefore, our work suggests that MEG could represent a noninvasive method for detecting cancer.

References

Dec 14, 1973·Nature·R J EricssonM Nishino
Jan 2, 1973·Biochimica Et Biophysica Acta·Y OkadaK Izutsu
Apr 4, 1980·Science·J P WikswoJ A Freeman
Oct 16, 1999·The EMBO Journal·L A PardoW Stühmer
Jun 5, 2001·Nature Reviews. Neuroscience·D E ClaphamC Strübing
Dec 5, 2003·Nature·David E Clapham
May 5, 2004·The Journal of Clinical Investigation·Laszlo KaraiMichael J Iadarola
Nov 17, 2004·Advances in Physiology Education·Stephen H Wright
Dec 20, 2005·The Journal of Membrane Biology·Karl Kunzelmann
Oct 7, 2006·Molecular Cancer·Fernanda Mello de QueirozLuis A Pardo
Aug 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·John V Frangioni
Feb 20, 2010·Trends in Molecular Medicine·Natalia PrevarskayaYaroslav Shuba
Apr 21, 2010·Experimental Biology and Medicine·Ranjith S Wijesinghe
Oct 5, 2010·Nature Reviews. Drug Discovery·Ramiro GarzonCarlo M Croce
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Liam T HallLloyd C L Hollenberg
Sep 21, 2012·Channels·William J Brackenbury
Apr 27, 2013·Nature·D Le SageR L Walsworth
Feb 24, 2017·Nature Neuroscience·Sylvain Baillet
Aug 9, 2017·Proceedings of the National Academy of Sciences of the United States of America
Feb 24, 2018·Science·Mark Kalinich, Daniel A Haber

❮ Previous
Next ❯

Citations

Jun 18, 2020·International Journal of Molecular Sciences·Kevin ZhaiDietrich Büsselberg

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
Fluorescence Microscopy
florescence imaging
transfection

Software Mentioned

MatLab
Prism
Image J - Fiji
FluoView
Origin Pro
DeltaGraph
MEG 160
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annals of Indian Academy of Neurology
Sanjay P Singh
Methods in Molecular Biology
Andreas A Ioannides
Handbook of Clinical Neurology
Ritva Paetau, Ismail S Mohamed
Rinshō shinkeigaku = Clinical neurology
N Nakasato
© 2021 Meta ULC. All rights reserved