Measuring degranulation of mast cells

Current Protocols in Immunology
R J Hohman, S C Dreskin

Abstract

This unit describes methods for measuring exocytosis of preformed mediators from secretory granules as an indication of IgE receptor-mediated activation of mast cells. The first basic protocol describes the measurement of biogenic amines (serotonin and histamine) secreted by activated rodent mast cells (for serotonin) or rodent and human mast cells (for histamine). The second basic and alternate protocols detail techniques for measuring the release of beta-glucuronidase, an enzyme that is synthesized by human and rodent mast cells, stored in secretory granules, and released during degranulation. Methods for assaying other enzymes released during degranulation, such as beta-hexosaminidase and tryptase, are discussed in the . These protocols can also be applied to basophils where appropriate.

References

Oct 1, 1990·The Journal of Allergy and Clinical Immunology·L B Schwartz
Mar 1, 1967·Clinica Chimica Acta; International Journal of Clinical Chemistry·W H FishmanS Green

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Citations

Dec 3, 2014·Frontiers in Immunology·Tae Chul MoonMarianna Kulka
Sep 10, 2014·The Journal of Allergy and Clinical Immunology·Tina L SumpterAdriana T Larregina
Apr 10, 2012·Immunologic Research·Madhan MasilamaniHugh A Sampson
Jan 26, 2018·Clinical Reviews in Allergy & Immunology·Quindelyn S Cook, A Wesley Burks
Jun 6, 2020·Journal of Immunoassay & Immunochemistry·Muhammad Novrizal Abdi Sahid, Takeshi Kiyoi
Jun 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yokananth SekarA Dean Befus

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