Measuring Escherichia coli Gene Expression during Human Urinary Tract Infections.

Pathogens
Harry L T Mobley

Abstract

Extraintestinal Escherichia coli (E. coli) evolved by acquisition of pathogenicity islands, phage, plasmids, and DNA segments by horizontal gene transfer. Strains are heterogeneous but virulent uropathogenic isolates more often have specific fimbriae, toxins, and iron receptors than commensal strains. One may ask whether it is the virulence factors alone that are required to establish infection. While these virulence factors clearly contribute strongly to pathogenesis, bacteria must survive by metabolizing nutrients available to them. By constructing mutants in all major metabolic pathways and co-challenging mice transurethrally with each mutant and the wild type strain, we identified which major metabolic pathways are required to infect the urinary tract. We must also ask what else is E. coli doing in vivo? To answer this question, we examined the transcriptome of E. coli CFT073 in the murine model of urinary tract infection (UTI) as well as for E. coli strains collected and analyzed directly from the urine of patients attending either a urology clinic or a university health clinic for symptoms of UTI. Using microarrays and RNA-seq, we measured in vivo gene expression for these uropathogenic E. coli strains, identifying genes ...Continue Reading

References

Jul 1, 1988·Reviews of Infectious Diseases·S Falkow
Dec 19, 2000·Annals of Epidemiology·B FoxmanJ D Sobel
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Andrew T RevelMichael V Norgard
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·R A WelchF R Blattner
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Qing XuJohn J Mekalanos
May 20, 2003·The Journal of Biological Chemistry·Kirsty SalmonRobert P Gunsalus
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juliano TimmJohn D McKinney
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Adel M TalaatStephen Albert Johnston
Oct 27, 2004·Infection and Immunity·Jennifer A SnyderHarry L T Mobley
Mar 3, 2005·Journal of Molecular Microbiology and Biotechnology·Diana L WilliamsThomas P Gillis
Aug 29, 2006·The American Journal of Pathology·Morag R GrahamJames M Musser
Oct 24, 2006·PLoS Pathogens·Alex Toftgaard NielsenGary K Schoolnik
Mar 14, 2007·Journal of Bacteriology·Amanda L LloydHarry L T Mobley
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·David R ScottGeorge Sachs
Oct 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·M Chelsea LaneHarry L T Mobley
Dec 12, 2007·Infection and Immunity·Melissa L MadsenF Chris Minion
Mar 31, 2009·Journal of Bacteriology·Amanda L LloydHarry L T Mobley
Sep 12, 2012·Infection and Immunity·Rachel R SpurbeckHarry L T Mobley
Dec 18, 2013·PLoS Pathogens·Sargurunathan SubashchandraboseHarry L T Mobley
Dec 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sargurunathan SubashchandraboseHarry L T Mobley

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Citations

Oct 18, 2019·Clinical Microbiology Reviews·Larry Reitzer, Philippe Zimmern
Apr 26, 2017·BMC Microbiology·Michelle MadelungJakob Møller-Jensen
Mar 27, 2020·MBio·Rajdeep BanerjeePatricia J Kiley
Aug 10, 2021·Frontiers in Cellular and Infection Microbiology·Jonathan Josephs-SpauldingChristoph Kaleta

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Methods Mentioned

BETA
RNA-seq
Illumina sequencing
RNAseq

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