Measuring Local Viscosities near Plasma Membranes of Living Cells with Photonic Force Microscopy

Biophysical Journal
Felix JüngerAlexander Rohrbach

Abstract

The molecular processes of particle binding and endocytosis are influenced by the locally changing mobility of the particle nearby the plasma membrane of a living cell. However, it is unclear how the particle's hydrodynamic drag and momentum vary locally and how they are mechanically transferred to the cell. We have measured the thermal fluctuations of a 1 μm-sized polystyrene sphere, which was placed in defined distances to plasma membranes of various cell types by using an optical trap and fast three-dimensional (3D) interferometric particle tracking. From the particle position fluctuations on a 30 μs timescale, we determined the distance-dependent change of the viscous drag in directions perpendicular and parallel to the cell membrane. Measurements on macrophages, adenocarcinoma cells, and epithelial cells revealed a significantly longer hydrodynamic coupling length of the particle to the membrane than those measured at giant unilamellar vesicles (GUVs) or a plane glass interface. In contrast to GUVs, there is also a strong increase in friction and in mean first passage time normal to the cell membrane. This hydrodynamic coupling transfers a different amount of momentum to the interior of living cells and might serve as an u...Continue Reading

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Citations

Jul 11, 2016·The Journal of Chemical Physics·Abdallah Daddi-Moussa-Ider, Stephan Gekle
Feb 7, 2018·The European Physical Journal. E, Soft Matter·Abdallah Daddi-Moussa-Ider, Stephan Gekle
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Feb 18, 2017·Physical Review. E·Abdallah Daddi-Moussa-Ider, Stephan Gekle
Feb 13, 2016·Physical Review. E·Abdallah Daddi-Moussa-IderStephan Gekle
Jul 10, 2018·The Journal of Chemical Physics·Abdallah Daddi-Moussa-IderHartmut Löwen
Jan 8, 2019·Biomedical Optics Express·Anjan Bhat KashekodiAlexander Rohrbach
Mar 12, 2021·Scientific Reports·Andrew B MathesonPaul A Dalgarno

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