Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

Nature Communications
Shaolin LiangJulian A Tanner

Abstract

Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

References

Jun 8, 2001·Annals of Clinical Biochemistry·M J Wheeler
Sep 22, 2005·The Journal of Clinical Endocrinology and Metabolism·Waljit S DhilloStephen R Bloom
Oct 10, 2007·Journal of Endocrinological Investigation·A BachelotF Kuttenn
Apr 3, 2009·Journal of Medical Engineering & Technology·D E HenleyS L Lightman
Jun 28, 2011·BMC Systems Biology·Emma GranqvistRichard J Morris
Aug 29, 2012·Bio Systems·Emma GranqvistRichard J Morris
Jan 29, 2013·Applied Biochemistry and Biotechnology·Anish KhanSulaiman Ab Ghani
Sep 3, 2013·Methods in Molecular Biology·Michael J Wheeler
Nov 29, 2013·Science Translational Medicine·Brian Scott FergusonHyongsok Tom Soh
May 2, 2014·Methods in Molecular Biology·Robert L Barbieri
May 6, 2015·Annual Review of Analytical Chemistry·Elizabeth S Bucher, R Mark Wightman
Jul 15, 2015·Annual Review of Analytical Chemistry·Megan C Frost, Mark E Meyerhoff
Apr 14, 2016·Annual Review of Analytical Chemistry·Lauren R Schoukroun-BarnesRyan J White
Oct 8, 2016·Endocrinology·Katja CzieselskyAllan E Herbison
Dec 15, 2016·Journal of the American Chemical Society·Hui LiKevin W Plaxco
Jan 11, 2017·Proceedings of the National Academy of Sciences of the United States of America·Netzahualcóyotl Arroyo-CurrásKevin W Plaxco
Oct 3, 2017·Diabetes & Metabolic Syndrome·David Olczuk, Ronny Priefer

❮ Previous
Next ❯

Citations

May 1, 2019·Advanced Biosystems·Lewis A FraserJulian A Tanner
Jul 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yee-Wai CheungJulian A Tanner
Oct 11, 2020·International Journal of Molecular Sciences·Chioma Izzi-EngbeayaWaljit S Dhillo
Dec 1, 2020·Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia·Gabriel Acácio de Moura, Paula Bruno Monteiro
Apr 17, 2021·Frontiers in Physiology·Richard Ivell, Ravinder Anand-Ivell
May 20, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Zihan GaoHao Li
Jun 24, 2021·Nature Reviews. Microbiology·Timothy M RawsonAlison H Holmes
Jun 29, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alexander MacdonaldDamion K Corrigan
Jan 27, 2022·Journal of Neuroendocrinology·Margaritis VoliotisKrasimira Tsaneva-Atanasova

❮ Previous
Next ❯

Methods Mentioned

BETA
SELEX
electrophoresis
electrophoretic mobility shift assay
surface plasmon resonance
circular dichroism
biosensor
membrane filtration
blood collection
PCR
chip

Software Mentioned

R
Excel
BSA
Opentron
ELONA
PSTrace
MedCal
Origin
MFold
RAPTER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Chenggang Wang, Zhihong Mo
Angewandte Chemie
Itamar Willner, Maya Zayats
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Hongru LiangXianzhu Xia
Mini Reviews in Medicinal Chemistry
Ke-Tai GuoHans P Wendel
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Lian-Hua QinZhong-Yi Hu
© 2022 Meta ULC. All rights reserved