Measuring maximum and standard metabolic rates using intermittent-flow respirometry: a student laboratory investigation of aerobic metabolic scope and environmental hypoxia in aquatic breathers

Journal of Fish Biology
P J RosewarneJon C Svendsen

Abstract

Metabolic rate is one of the most widely measured physiological traits in animals and may be influenced by both endogenous (e.g. body mass) and exogenous factors (e.g. oxygen availability and temperature). Standard metabolic rate (SMR) and maximum metabolic rate (MMR) are two fundamental physiological variables providing the floor and ceiling in aerobic energy metabolism. The total amount of energy available between these two variables constitutes the aerobic metabolic scope (AMS). A laboratory exercise aimed at an undergraduate level physiology class, which details the appropriate data acquisition methods and calculations to measure oxygen consumption rates in rainbow trout Oncorhynchus mykiss, is presented here. Specifically, the teaching exercise employs intermittent flow respirometry to measure SMR and MMR, derives AMS from the measurements and demonstrates how AMS is affected by environmental oxygen. Students' results typically reveal a decline in AMS in response to environmental hypoxia. The same techniques can be applied to investigate the influence of other key factors on metabolic rate (e.g. temperature and body mass). Discussion of the results develops students' understanding of the mechanisms underlying these fundame...Continue Reading

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Citations

Apr 1, 2016·The Journal of Experimental Biology·Mark J GarciaRyan L Earley
Jun 27, 2020·The Journal of Experimental Biology·Jessica E Reemeyer, Bernard B Rees
Sep 19, 2019·The Journal of Experimental Biology·S Clay SteellErika J Eliason
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Mar 10, 2020·Toxicon : Official Journal of the International Society on Toxinology·Mauro CorriereLucía Soliño
Oct 15, 2021·The Journal of Experimental Biology·Emily A HardisonErika J Eliason

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