Measuring maximum oxygen uptake with an incremental swimming test and by chasing rainbow trout to exhaustion inside a respirometry chamber yields the same results

Journal of Fish Biology
Yangfan ZhangAnthony P Farrell

Abstract

This study hypothesized that oxygen uptake (ṀO2 ) measured with a novel protocol of chasing rainbow trout Oncorhynchus mykiss to exhaustion inside a static respirometer while simultaneously monitoring ṀO2 (ṀO2chase ) would generate the same and repeatable peak value as when peak active ṀO2 (ṀO2active ) is measured in a critical swimming speed protocol. To reliably determine peak ṀO2chase , and compare to the peak during recovery of ṀO2 after a conventional chase protocol outside the respirometer (ṀO2rec ), this study applied an iterative algorithm and a minimum sampling window duration (i.e., 1 min based on an analysis of the variance in background and exercise ṀO2 ) to account for ṀO2 dynamics. In support of this hypothesis, peak ṀO2active (707 ± 33 mg O2 h-1 kg-1 ) and peak ṀO2chase (663 ± 43 mg O2 h-1 kg-1 ) were similar (P = 0.49) and repeatable (Pearson's and Spearman's correlation test; r ≥ 0.77; P < 0.05) when measured in the same fish. Therefore, estimates of ṀO2max can be independent of whether a fish is exhaustively chased inside a respirometer or swum to fatigue in a swim tunnel, provided ṀO2 is analysed with an iterative algorithm and a minimum but reliable sampling window. The importance of using this analytical ap...Continue Reading

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Citations

Aug 7, 2021·Conservation Physiology·Graham D RabyAaron T Fisk
Sep 15, 2021·The Journal of Experimental Biology·Shaun S KillenDavid J McKenzie

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