Measuring mechanical properties, including isotonic fatigue, of fast and slow MLC/mIgf-1 transgenic skeletal muscle

Annals of Biomedical Engineering
Zaccaria Del PreteEmanuele Rizzuto

Abstract

Contractile properties of fast-twitch (EDL) and slow-twitch (soleus) skeletal muscles were measured in MLC/mIgf-1 transgenic and wild-type mice. MLC/mIgf-1 mice express the local factor mIgf-1 under the transcriptional control of MLC promoter, selectively activated in fast-twitch muscle fibers. Isolated muscles were studied in vitro in both isometric and isotonic conditions. We used a rapid "ad hoc" testing protocol that measured, in a single procedure, contraction time, tetanic force, Hill's (F-v) curve, power curve and isotonic muscle fatigue. Transgenic soleus muscles did not differ from wild-type with regard to any measured variable. In contrast, transgenic EDL muscles displayed a hypertrophic phenotype, with a mass increase of 29.2% compared to wild-type. Absolute tetanic force increased by 21.5% and absolute maximum power by 34.1%. However, when normalized to muscle cross-sectional area and mass, specific force and normalized power were the same in transgenic and wild-type EDL muscles, revealing that mIgf-1 expression induces a functional hypertrophy without altering fibrotic tissue accumulation. Isotonic fatigue behavior did not differ between transgenic and wild-type muscles, suggesting that the ability of mIgf-1 transg...Continue Reading

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Citations

Feb 16, 2016·Journal of Nutritional Science and Vitaminology·Tomoya KitakazeRyoichi Yamaji
Mar 13, 2013·Scientific Reports·Silvia CarosioAntonio Musarò
Aug 8, 2015·Human Molecular Genetics·Laura PelosiAntonio Musarò
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Dec 3, 2008·Cell Metabolism·Gabriella DobrowolnyAntonio Musarò
Nov 14, 2017·Journal of Cachexia, Sarcopenia and Muscle·Francesca MolinariElisabetta Ferraro
Jan 30, 2015·Annals of Biomedical Engineering·Emanuele RizzutoZaccaria Del Prete
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Apr 7, 2019·Aging Cell·Francesca AscenziAntonio Musarò
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Jul 25, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Morten MunkvikOle M Sejersted
Jan 14, 2020·Nature Communications·Jessica SegalésPura Muñoz-Cánoves
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Laura ForcinaAntonio Musarò

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