Measuring serum concentrations of interleukin-33 in atopic dermatitis is associated with potential false positive results

SpringerPlus
Uffe NygaardMalene Hvid

Abstract

In the search for valid biomarkers in inflammatory diseases, cytokine serum concentrations are often measured by enzyme-linked immunosorbent assay and correlated to disease activity. Interleukin-33 is a relatively newly described cytokine, which holds a promising potential as a biomarker for different diseases including atopic dermatitis. However, interfering human anti-animal IgG antibodies and heterophilic antibodies might give rise to false positive or negative results that often go unnoticed. We performed a three-step validation of commercially available and widely used human interleukin-33 enzyme-linked immunosorbent assay kit with serum samples from eight atopic dermatitis patients and five healthy controls. Through addition of unspecific animal IgG (rabbit, mouse, goat and bovine) and unspecific human IgG to the assay diluent, we disclosed false positive values in 12 out of 13 samples. This study show that the present human interleukin-33 enzyme-linked immunosorbent assay kit might give rise to a high prevalence of false positive values if not validated. This inaccuracy is easily eliminated with a simple set of validation steps.

References

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Feb 27, 2014·Journal of the American Academy of Dermatology·Risa Tamagawa-MineokaNorito Katoh

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Citations

Oct 25, 2016·Journal of the European Academy of Dermatology and Venereology : JEADV·U NygaardC Vestergaard
Jul 2, 2016·Pharmacology & Therapeutics·Patrick D Mitchell, Paul M O'Byrne
Apr 6, 2016·AIDS·Vikram MehrajUNKNOWN Montreal Primary HIV Infection, the Canadian Long-Term Non-Progressors Study Groups
Apr 8, 2017·International Journal of Molecular Sciences·Wei-Yu ChenJenq-Lin Yang
Mar 14, 2018·Journal of the European Academy of Dermatology and Venereology : JEADV·A WollenbergC Vestergaard
Oct 12, 2018·Biochemical Society Transactions·Ian C ScottE Suzanne Cohen

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Methods Mentioned

BETA
ELISA

Software Mentioned

GraphPad
GraphPad Prism
STATA

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Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.