Measuring Size, Size Distribution, and Polydispersity of Water-in-Oil Microemulsion Droplets using Fluorescence Correlation Spectroscopy: Comparison to Dynamic Light Scattering

The Journal of Physical Chemistry. B
Mohammad Firoz KhanSobhan Sen

Abstract

Water-in-oil microemulsion droplets (MEDs) are thermodynamically stable supramolecular structures formed in a mixture of water and oil, stabilized by surfactant layer. Here we use fluorescence correlation spectroscopy (FCS) to measure the diffusion, and the size, size distribution, and polydispersity of MEDs prepared in ternary mixtures of water/oil/sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in heptane, isooctane, and nonane at (near) single droplet level. We compare FCS data directly to dynamic light scattering (DLS) data, which shows that the optical matching point (OMP) conditions of MEDs in different oils (where excess optical polarizability of droplets vanish) severely influence DLS data, while FCS extracts the accurate size, size distribution, and polydispersity of AOT-MEDs in all three oils. This suggests that extreme precaution must be taken in acquiring and explaining DLS data of MEDs in solution. FCS data show nearly identical W0-dependent (peak) size variations of AOT-MEDs in all three oils, though a subtle increase in (average) polydispersity of droplets is observed with increase in carbon chain length of oils. Establishing the accuracy of FCS data for AOT-MEDs, we further apply FCS to measure the size parameters...Continue Reading

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Citations

Sep 27, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Aparajita PhukonKalyanasis Sahu
Dec 23, 2016·The Journal of Physical Chemistry. B·Muhammad Faizan NazarMuhammad Zubair
Feb 22, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Johanna MideletMartinus H V Werts
Jan 15, 2019·Journal of Colloid and Interface Science·Brian FuglestadKim A Sharp
Jul 22, 2019·The Science of the Total Environment·Jared ChurchDanielle M Paynter
Aug 16, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Muhammad Faizan NazarAzwan Mat Lazim
Sep 22, 2018·The Journal of Physical Chemistry. B·Benjamin P Wiebenga-SanfordNancy E Levinger
Dec 31, 2020·The Journal of Physical Chemistry. B·Kristin BryantSteven R Saunders
Jun 20, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Muhammad Atif SaleemMazlan Mohamed
Apr 25, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Benoit RichardAnna M Ritcey
Oct 14, 2021·Analytical Chemistry·Dai KatoMasashi Kunitake

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