Measuring specificity in multi-substrate/product systems as a tool to investigate selectivity in vivo

Biochimica Et Biophysica Acta
Yin-Ming KuoAndrew J Andrews

Abstract

Multiple substrate enzymes present a particular challenge when it comes to understanding their activity in a complex system. Although a single target may be easy to model, it does not always present an accurate representation of what that enzyme will do in the presence of multiple substrates simultaneously. Therefore, there is a need to find better ways to both study these enzymes in complicated systems, as well as accurately describe the interactions through kinetic parameters. This review looks at different methods for studying multiple substrate enzymes, as well as explores options on how to most accurately describe an enzyme's activity within these multi-substrate systems. Identifying and defining this enzymatic activity should help clear the way to using in vitro systems to accurately predicting the behavior of multi-substrate enzymes in vivo. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions.

References

Nov 5, 1991·Biochemistry·B A HorensteinV L Schramm
Nov 19, 1974·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A R Fersht
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A Levitzki, D E Koshland
Sep 1, 1970·Biotechnology and Bioengineering·V H Edwards
Sep 1, 1984·The Biochemical Journal·M L CárdenasH Niemeyer
Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Allende, C C Allende
Apr 27, 1993·Biochemistry·V SchellenbergerW J Rutter
Aug 1, 1996·BioTechniques·K Zimmermann, J W Mannhalter
Mar 1, 1997·Nature Medicine·J A Monforte, C H Becker
Sep 1, 1996·Nature Biotechnology·H KösterC R Cantor
Feb 20, 1999·The Journal of Biological Chemistry·P A GrantJ L Workman
Oct 12, 1999·Biotechnology and Bioengineering·S GuhaP R Jaffé
May 16, 2000·The Journal of Biological Chemistry·K G TannerJ M Denu
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D E Sterner, S L Berger
Jan 4, 2001·Drug Metabolism Reviews·S A WrightonP B Watkins
Jun 27, 2002·European Journal of Biochemistry·Michael J Knapp, Judith P Klinman
Apr 23, 2003·Trends in Pharmacological Sciences·Sean EkinsJ Andrew Williams
Apr 1, 1963·Journal of Molecular Biology·J MONODF JACOB
Jan 1, 1965·Annual Review of Biochemistry·M L BENDER, J KEZDY
Nov 6, 2003·Journal of the American Chemical Society·Corbin J ZeaNicola L Pohl
Feb 10, 2004·Journal of the American Society for Mass Spectrometry·Na Pi, Julie A Leary
Dec 8, 2004·Archives of Biochemistry and Biophysics·W W Cleland
Feb 3, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jian-Qiu HanDavid J Barton
Feb 26, 2005·The Journal of Organic Chemistry·Kwang-Seuk KoNicola L Pohl
Apr 15, 2005·Mutation Research·John R EdwardsJingyue Ju
Jul 12, 2005·Analytical and Bioanalytical Chemistry·André Liesener, Uwe Karst
Jul 20, 2005·Biochimica Et Biophysica Acta·Cindy HuntPaul C Kline
Oct 15, 2005·Nature Reviews. Drug Discovery·Larry C Wienkers, Timothy G Heath
Feb 6, 2007·Biochemistry·Christopher E BerndsenJohn M Denu
Feb 21, 2009·The FEBS Journal·James J Champoux, Sharon J Schultz
Apr 27, 2010·Nature Chemical Biology·Jefferson ChanAndrew J Bennet

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