Measuring the ability of HIV-specific antibodies to mediate trogocytosis

Journal of Immunological Methods
Simone I RichardsonLynn Morris

Abstract

Antibody Fc effector functions contribute to HIV control and have been implicated in the partial efficacy seen in the RV144 vaccine trial. Fc-mediated trogocytosis has been previously described for anti-cancer antibodies and results in the removal of membrane fragments from target cells. Here we developed a flow cytometry-based assay which measures the transfer of membrane fragments from a gp120-coated CD4+ lymphocytic cell line (CEM.NKR-CCR5 cells stained with a membrane dye PKH26) to monocytic cells (THP-1 cells stained with CFSE). We showed that this transfer occurred rapidly, within 1 h, and was mediated through engagement of the FcγRIIa/b receptors on the THP-1 cells. HIV-specific IgG as well as gp120 and CD4 could be detected on the surface of THP-1 cells in a process that we demonstrated was distinct from phagocytosis. Furthermore, while the THP-1 effector cells remained intact following the receipt of new membrane proteins, the viability of the target CEM.NKR-CCR5 cells decreased over time. Analysis of HIV-specific plasma revealed that antibodies with trogocytic activity were common in acute and chronic HIV infection but were higher in individuals with broadly neutralizing antibody responses We also examined trogocytosi...Continue Reading

Citations

Apr 30, 2019·Current Opinion in HIV and AIDS·Simone I Richardson, Penny L Moore
Jul 18, 2020·Current Opinion in HIV and AIDS·Margaret C Carpenter, Margaret E Ackerman
Mar 16, 2019·Frontiers in Immunology·Matthew Zirui TayJustin Pollara
May 28, 2019·Frontiers in Immunology·George K LewisBargavi Thyagarajan
Feb 16, 2021·The Journal of Infectious Diseases·Evan RossignolBoris Julg
Mar 24, 2021·Expert Opinion on Therapeutic Targets·Simone I Richardson, Penny L Moore
May 18, 2021·Cell Reports Medicine·Wen Shi LeeStephen J Kent
Jun 1, 2021·Cell Reports Medicine·Andrea R ShiakolasIvelin S Georgiev
Sep 30, 2021·Journal of Virology·Lauren NagelUNKNOWN investigators in Montreal Primary HIV Infection cohort

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