Measuring the Genetic Diversity of Xanthomonas axonopodis pv. manihotis Within Different Fields in Colombia

Phytopathology
Silvia RestrepoValérie Verdier

Abstract

ABSTRACT Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis, is a widespread disease that affects cassava (Manihot esculenta). We collected 238 X. axonopodis pv. manihotis strains by intensively sampling single fields in four edaphoclimatic zones (ECZs) in Colombia. DNA polymorphism of different X. axonopodis pv. manihotis populations was assessed by restriction fragment length polymorphism (RFLP) analyses, repetitive sequence-based polymerase chain reaction (rep-PCR), and amplified fragment length polymorphism (AFLP) assays. Genetic diversity, phenetic relationships among strains, and the coefficient of genetic differentiation were determined. All strains were tested for aggressiveness on the susceptible cassava cv. MCOL 1522. Strains were also tested for virulence on cassava differentials adapted to the strains' respective ECZs. Our study showed that the Colombian X. axonopodis pv. manihotis population has a high degree of genetic diversity. The hierarchical analysis of diversity showed genotypic differentiation at all levels, among ECZs, among fields within ECZs, and among strains within fields planted to several cassava genotypes. New RFLP haplotypes were detected, leading to the characterization of a ...Continue Reading

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Citations

Oct 24, 2008·Phytopathology·Helvécio Della Coletta-Filho, Marcos Antonio Machado
Jun 15, 2016·Annual Review of Phytopathology·Marie-Agnès JacquesChristian Vernière
Jun 8, 2012·Molecular Plant Pathology·John MansfieldGary D Foster
Jun 12, 2019·Cold Spring Harbor Perspectives in Biology·Z J Daniel LinRebecca Bart
Jan 9, 2004·Applied and Environmental Microbiology·Silvia RestrepoValérie Verdier
Jul 31, 2002·Applied and Environmental Microbiology·Helvécio Della Coletta-Filho, Marcos Antonio Machado

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