Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma.

Analytical and Bioanalytical Chemistry
Adam M HawkridgeDavid C Muddiman

Abstract

The domestic chicken (Gallus domesticus) has emerged as a powerful experimental model for studying the onset and progression of spontaneous epithelial ovarian cancer (EOC) with a disease prevalence that can exceed 35% between 2 and 7 years of age. An experimental strategy for biomarker discovery is reported herein that combines the chicken model of EOC, longitudinal plasma sample collection with matched tissues, advanced mass spectrometry-based proteomics, and concepts derived from the index of individuality (Harris, Clin Chem 20: 1535-1542, 1974). Blood was drawn from 148 age-matched chickens starting at 2.5 years of age every 3 months for 1 year. At the conclusion of the 1 year sample collection period, the 73 birds that remained alive were euthanized, necropsied, and tissues were collected. Pathological assessment of resected tissues from these 73 birds confirmed that five birds (6.8%) developed EOC. A proteomics workflow including in-gel digestion, nanoLC coupled to high-performance mass spectrometry, and label-free (spectral counting) quantification was used to measure the biological intra-individual variability (CV(W)) of the chicken plasma proteome. Longitudinal plasma sample sets from two birds within the 73-bird biorep...Continue Reading

References

Aug 1, 1987·Environmental Health Perspectives·T N Fredrickson
Jul 17, 1971·Lancet·M F Fathalla
Oct 13, 1983·The New England Journal of Medicine·R C BastR C Knapp
Nov 1, 1981·The Journal of Clinical Investigation·R C BastR C Knapp
Jan 1, 1997·Cellular and Molecular Life Sciences : CMLS·I Saxena, S Tayyab
Jun 14, 2000·Animal Reproduction Science·E Ropstad
Jun 24, 2000·Journal of Reproduction and Fertility·M B Renfree, D R Blanden
Feb 24, 2001·Scandinavian Journal of Clinical and Laboratory Investigation·M K TuxenP Dombernowsky
Apr 11, 2001·Endocrine Reviews·N AuerspergP C Leung
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
Nov 26, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·N AuerspergG W E Mitchell
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Mar 21, 2003·Journal of Proteome Research·Dirk Chelius, Pavel V Bondarenko
Oct 28, 2003·Reproductive Biology and Endocrinology : RB&E·Dusica Cvetkovic
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Barbara C VanderhydenJean-François Ethier
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jan 30, 2004·Cancer Cell·Robert F OzolsThomas C Hamilton
Mar 5, 2004·Cancer Research·Jinsong LiuRobert C Bast
Mar 5, 2004·Breast Cancer Research and Treatment·Mark H KaplanMichael H Dosik
Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Jun 24, 2004·Seminars in Cell & Developmental Biology·Ramon Parsons
Dec 14, 2004·Molecular & Cellular Proteomics : MCP·Diana M Smith-BeckermanAlma L Burlingame
Jan 15, 2005·Analytical Chemistry·Jacques ColingeLydie Bougueleret
Feb 11, 2005·Molecular & Cellular Proteomics : MCP·D Brent WeatherlyRon Orlando
Jun 16, 2005·Molecular and Cellular Endocrinology·Kenneth GarsonBarbara C Vanderhyden
Jun 28, 2005·Molecular & Cellular Proteomics : MCP·William M OldNatalie G Ahn
Aug 9, 2005·Journal of Proteome Research·Richard E HiggsJohn E Hale
Aug 27, 2005·Proteomics·Sergei A MoshkovskiiAlexander I Archakov
Jan 13, 2006·Nature Chemical Biology·Shao-En Ong, Matthias Mann

❮ Previous
Next ❯

Citations

Apr 25, 2012·Analytical and Bioanalytical Chemistry·Michael S BeremanMichael J MacCoss
Oct 8, 2011·Reproductive Biology and Endocrinology : RB&E·Whasun LimGwonhwa Song
Feb 9, 2017·Journal of Proteome Research·James McCordDavid C Muddiman
Apr 8, 2016·Proteomics Insights·Balamurugan PackialakshmiNarayan C Rath
May 17, 2013·Nature Reviews. Cancer·Patricia A Johnson, James R Giles
Aug 19, 2014·Proteomics. Clinical Applications·Adam M Hawkridge
Aug 6, 2011·Rapid Communications in Mass Spectrometry : RCM·Timothy S CollierDavid C Muddiman
Apr 19, 2011·Analytical Chemistry·Xudong Yao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.