Measuring the lactate-to-creatine ratio via 1 H NMR spectroscopy can be used to noninvasively evaluate apoptosis in glioma cells after X-ray irradiation

Cellular & Molecular Biology Letters
Hongxia LiQingshi Zeng


Radiotherapy is among the commonly applied treatment options for glioma, which is one of the most common types of primary brain tumor. To evaluate the effect of radiotherapy noninvasively, it is vital for oncologists to monitor the effects of X-ray irradiation on glioma cells. Preliminary research had showed that PKC-ι expression correlates with tumor cell apoptosis induced by X-ray irradiation. It is also believed that the lactate-to-creatine (Lac/Cr) ratio can be used as a biomarker to evaluate apoptosis in glioma cells after X-ray irradiation. In this study, we evaluated the relationships between the Lac/Cr ratio, apoptotic rate, and protein kinase C iota (PKC-ι) expression in glioma cells. Cells of the glioma cell lines C6 and U251 were randomly divided into 4 groups, with every group exposed to X-ray irradiation at 0, 1, 5, 10 and 15 Gy. Single cell gel electrophoresis (SCGE) was conducted to evaluate the DNA damage. Flow cytometry was performed to measure the cell cycle blockage and apoptotic rates. Western blot analysis was used to detect the phosphorylated PKC-ι (p-PKC-ι) level. 1H NMR spectroscopy was employed to determine the Lac/Cr ratio. The DNA damage increased in a radiation dose-dependent manner (p < 0.05). With ...Continue Reading


May 27, 2006·Science·Satoaki MatobaPaul M Hwang
Jun 6, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Georg Martiny-Baron, Doriano Fabbro
Jul 10, 2007·Journal of Neuro-oncology·Qing-Shi ZengJun-Hui Zhen
Jan 24, 2008·Cell Proliferation·R PatelM Acevedo-Duncan
Nov 30, 2011·Cancer Research·Tanupriya Contractor, Chris R Harris
Jan 10, 2012·Cell Metabolism·Ubaldo E Martinez-OutschoornMichael P Lisanti
Jan 30, 2013·Cancer Biology & Therapy·Anália do CarmoMaria Celeste Lopes
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nadja BitomskyThomas G Hofmann
Mar 7, 2014·Investigative Ophthalmology & Visual Science·Jasmina Ziva CerneSean Eric McGuire
Jan 3, 2015·Biochimica Et Biophysica Acta·Joana Balça-SilvaMaria Celeste Lopes
Feb 28, 2015·Molecular Cancer Research : MCR·Uday Bhanu MaachaniAnita Tandle
Feb 28, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Johan Bussink, Paul N Span
Apr 22, 2015·International Journal of Oncology·Laura AnnovazziDavide Schiffer
Mar 1, 2016·Oxidative Medicine and Cellular Longevity·Shihoko Kimura-Ohba, Yi Yang

❮ Previous
Next ❯

Methods Mentioned

flow cytometry

Related Concepts

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2021 Meta ULC. All rights reserved