Measuring the simultaneous effects of hypoxia and deformation on ATP release from erythrocytes

The Analyst
Andrea Faris, Dana M Spence

Abstract

It is known that adenosine triphosphate (ATP) is released from red blood cells (RBCs) due to various forms of stimulation such as deformation, pharmacological stimuli, and hypoxia. To date, these various stimuli have been investigated individually. Here, we have combined a microflow system capable of initiating deformation-induced release of ATP from the RBCs at various levels of hypoxia as measured by percent oxygen saturation in the RBC sample. When values of ATP released from deformation and hypoxia are compared to values of ATP release due to hypoxia alone, the relationship between the two stimuli can be deduced. Measurement of RBC-derived ATP with the well-known chemiluminescence assay employing luciferin/luciferase indicates that RBCs deoxygenated for 4 min released 1.84 +/- 0.075 microM ATP. The largest decrease in oxygen saturation was found to be between 0 s (66.3% O(2) saturation) and 15 s (22.3% O(2) saturation). RBCs deoxygenated to a 22.3% O(2) saturation released 0.374 +/- 0.011 microM ATP when pumped through the microflow system. This value is an increase from 0.281 +/- 0.007 microM ATP in the presence of flow alone. The ATP release after exposure to hypoxia at 22.3% O(2) saturation was 0.381 +/- 0.014 microM ATP...Continue Reading

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Citations

Sep 21, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Stéphane P DufourJosé González-Alonso
Aug 5, 2010·Experimental Biology and Medicine·Meera SridharanAlan H Stephenson
Sep 22, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jiandi WanHoward A Stone
Aug 12, 2015·Purinergic Signalling·Geoffrey Burnstock
Sep 17, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Amit KumarDaya Shankar Pandey
Mar 23, 2010·Microvascular Research·Alison M ForsythHoward A Stone
Dec 3, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yimeng WangDana M Spence
Apr 26, 2014·Lab on a Chip·Jayda L ErkalDana M Spence
Jan 13, 2018·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Jordan E Mancuso, William D Ristenpart
Sep 6, 2018·PloS One·Jordan E MancusoWilliam D Ristenpart
Apr 25, 2020·The Journal of Physiology·Janée D TerwoordFrank A Dinenno
Nov 6, 2015·Critical Care : the Official Journal of the Critical Care Forum·Ryon M BatemanChristopher G Ellis
Jan 16, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Brett S KirbyTimothy J McMahon
Jun 25, 2021·Journal of Applied Physiology·Janée D TerwoordFrank A Dinenno

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