Meat, the metabolites: an integrated metabolite profiling and lipidomics approach for the detection of the adulteration of beef with pork

The Analyst
Drupad K TrivediRoyston Goodacre

Abstract

Adulteration of high quality food products with sub-standard and cheaper grades is a world-wide problem taxing the global economy. Currently, many traditional tests suffer from poor specificity, highly complex outputs and a lack of high-throughput processing. Metabolomics has been successfully used as an accurate discriminatory technique in a number of applications including microbiology, cancer research and environmental studies and certain types of food fraud. In this study, we have developed metabolomics as a technique to assess the adulteration of meat as an improvement on current methods. Different grades of beef mince and pork mince, purchased from a national retail outlet were combined in a number of percentage ratios and analysed using GC-MS and UHPLC-MS. These techniques were chosen because GC-MS enables investigations of metabolites involved in primary metabolism whilst UHPLC-MS using reversed phase chromatography provides information on lipophilic species. With the application of chemometrics and statistical analyses, a panel of differential metabolites were found for identification of each of the two meat types. Additionally, correlation was observed between metabolite content and percentage of fat declared on meat ...Continue Reading

References

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Citations

May 18, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Alberto Ruiz OrdunaFrancis Beaudry
Apr 17, 2018·Electrophoresis·Tamara MartinovićDjuro Josić
May 15, 2020·Metabolites·Susumu MuroyaPer Ertbjerg
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Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Tong SunChanghu Xue
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Yun-Cheng LiJia-Min Zhang

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Methods Mentioned

BETA
PCA
fatty acid assay

Software Mentioned

TunePlus
Matlab
DA
R
code
Xcalibur
MetaboAnalyst
PLS
ChromaTOF

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