Mechanical Adaptations of Epithelial Cells on Various Protruded Convex Geometries.

Cells
Sun-Min YuYoon-Kyoung Cho

Abstract

The shape of epithelial tissue supports physiological functions of organs such as intestinal villi and corneal epithelium. Despite the mounting evidence showing the importance of geometry in tissue microenvironments, the current understanding on how it affects biophysical behaviors of cells is still elusive. Here, we cultured cells on various protruded convex structure such as triangle, square, and circle shape fabricated using two-photon laser lithography and quantitatively analyzed individual cells. Morphological data indicates that epithelial cells can sense the sharpness of the corner by showing the characteristic cell alignments, which was caused by actin contractility. Cell area was mainly influenced by surface convexity, and Rho-activation increased cell area on circle shape. Moreover, we found that intermediate filaments, vimentin, and cytokeratin 8/18, play important roles in growth and adaptation of epithelial cells by enhancing expression level on convex structure depending on the shape. In addition, microtubule building blocks, α-tubulin, was also responded on geometric structure, which indicates that intermediate filaments and microtubule can cooperatively secure mechanical stability of epithelial cells on convex s...Continue Reading

References

Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Jan 9, 2004·The New England Journal of Medicine·Patricia D Wilson
Oct 3, 2009·Plant Physiology
Oct 22, 2009·Physical Biology·Y Y Biton, S A Safran
Jan 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melissa G MendezRobert D Goldman
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristopher A KilianMilan Mrksich
Mar 26, 2010·Journal of Cellular Biochemistry·Esther W GomezCeleste M Nelson
Nov 28, 2012·Advanced Healthcare Materials·Cécile M BidanJohn W C Dunlop
Oct 23, 2013·The Journal of Cell Biology·Benedetta CerrutiAndrea Gamba
Apr 14, 2015·Cell·Amy E ShyerClifford J Tabin
Apr 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hannah G YevickPascal Silberzan
Jul 15, 2015·Nature Communications·Zhen MaKevin E Healy
Apr 5, 2016·Nature Materials·Junmin LeeKristopher A Kilian
Nov 16, 2016·Scientific Reports·Giuseppe Antonacci, Sietse Braakman
Jan 14, 2017·Nature Communications·Julie SalomonDelphine Delacour
Feb 17, 2017·Journal of Cell Science·Craig S Nowell, Freddy Radtke
May 4, 2017·Proceedings of the National Academy of Sciences of the United States of America·Nancy CostigliolaGaudenz Danuser
Sep 30, 2017·Frontiers in Cell and Developmental Biology·Rucha Sanghvi-Shah, Gregory F Weber
Nov 17, 2017·Nature Communications·Wang XiChwee Teck Lim
Feb 13, 2018·Nature Materials·Thomas C von ErlachMolly M Stevens
Mar 29, 2018·Biophysical Journal·Nathan D BadeKathleen J Stebe
Jun 22, 2018·Chemical Reviews·Parthiv Kant ChaudhuriChwee Teck Lim
Jul 29, 2018·Nature Communications·Pedro Gómez-GálvezLuis M Escudero
Aug 22, 2018·Advanced Science·Min BaoWilhelm T S Huck
Dec 24, 2018·Journal of Cell Science·Florian A MaechlerCaterina Tomba
Mar 20, 2019·Trends in Biotechnology·Danielle BaptistaRoman Truckenmüller
Aug 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jiliang HuMing Guo
Aug 17, 2019·Science·Denis KrndijaDanijela Matic Vignjevic
Aug 28, 2019·Nature Materials·Tom P J WyattGuillaume T Charras
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joleen S CheahSoichiro Yamada
Sep 20, 2019·Science Advances·S EhrigJohn W C Dunlop

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
atomic force microscopy
AFM
fluorescence imaging
Fluorescence
Assay
GTPase

Software Mentioned

ZEN
SolidWorks
ImageJ
OriginPro
Lat
ITT visual
Gwyddion AFM
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Neurophysiology
Johannes HaushoferNancy Kanwisher
Discrete & Computational Geometry
Joshua CruzBill Kronholm
IEEE Transactions on Pattern Analysis and Machine Intelligence
C E Kim, A Rosenfeld
IEEE Transactions on Pattern Analysis and Machine Intelligence
Jovisa Zunic, Paul L Rosin
© 2022 Meta ULC. All rights reserved