Mechanical Characterization of the Lamellar Structure of Human Abdominal Aorta in the Development of Atherosclerosis: An Atomic Force Microscopy Study

Cardiovascular Engineering and Technology
Alireza Rezvani-SharifAlberto Avolio

Abstract

Atherosclerosis is a major risk factor for cardiovascular disease. However, mechanisms of interaction of atherosclerotic plaque development and local stiffness of the lamellar structure of the arterial wall are not well established. In the current study, the local Young's modulus of the wall and plaque components were determined for three different groups of healthy, mildly diseased and advanced atherosclerotic human abdominal aortas. Histological staining was performed to highlight the atherosclerotic plaque components and lamellar structure of the aortic media, consisting of concentric layers of elastin and interlamellar zones. The force spectroscopy mode of the atomic force microscopy was utilized to determine Young's moduli of aortic wall lamellae and plaque components at the micron level. The high variability of Young's moduli (E) at different locations of the atherosclerotic plaque such as the fibrous cap (E = 15.5± 2.6 kPa), calcification zone (E = 103.7±19.5 kPa), and lipid pool (E = 3.5±1.2 kPa) were observed. Reduction of elastin lamellae stiffness (18.6%), as well as stiffening of interlamellar zones (50%), were detected in the diseased portion of the medial layer of abdominal aortic wall compared to the healthy arte...Continue Reading

References

Jan 1, 1967·Circulation Research·H Wolinsky, S Glagov
Aug 1, 1994·Journal of Biomechanical Engineering·T Matsumoto, K Hayashi
Feb 9, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·M A CattellP S Hasleton
Jul 9, 1999·Journal of Biomedical Materials Research·J Y RhoG M Pharr
Apr 28, 2004·Journal of Biomechanics·Takeo MatsumotoMasaaki Sato
Jan 15, 2005·Journal of Biomechanical Engineering·Gerhard A HolzapfelPeter Regitnig
Feb 26, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Susan J ZiemanDavid A Kass
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Sep 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuliya VengrenyukSheldon Weinbaum
Jun 2, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Vyacheslav A KorshunovBradford C Berk
Jan 30, 2008·Annals of Biomedical Engineering·Brian H WaymanRaymond P Vito
Feb 16, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Amanda C DoranColeen A McNamara
Dec 25, 2008·Journal of Biomedical Materials Research. Part a·Donna M EbensteinLisa A Pruitt
Apr 4, 2009·Journal of Structural Biology·Julie A LastChristopher J Murphy
Sep 22, 2009·Journal of Biomechanics·Eoghan MaherDaniel J Kelly
Sep 30, 2010·Journal of Medical Engineering & Technology·Nooshin HaghighipourAlbert Avolio
Feb 4, 2011·Journal of Biomechanics·John PeloquinCynthia A Reinhart-King
Feb 8, 2011·Journal of Structural Biology·Philippe TracquiLaurent Riou
Feb 10, 2011·Tissue Engineering. Part B, Reviews·Clayton T McKeeChristopher J Murphy
Jan 1, 2008·CSH Protocols·Andrew H FischerRolf Zeller
Dec 14, 2011·Science Translational Medicine·John HuynhCynthia A Reinhart-King
Jan 3, 2012·Archives of Biochemistry and Biophysics·Małgorzata LekkaPiotr Laidler
Feb 18, 2014·Journal of Biomechanics·Ali C AkyildizFrank J H Gijsen
Apr 9, 2014·Biomechanics and Modeling in Mechanobiology·Alexey V KamenskiyJason N MacTaggart
Dec 9, 2014·Journal of the Mechanical Behavior of Biomedical Materials·Siobhan A O'LearyBarry J Doyle
Jul 15, 2015·IEEE Transactions on Medical Imaging·Iason Zacharias ApostolakisElisa E Konofagou
Sep 16, 2015·Journal of the American Society of Hypertension : JASH·Hadi TaghizadehMohammad B Shadmehr
Apr 1, 2013·Pulse·Alberto Avolio
Aug 2, 2016·Journal of the Mechanical Behavior of Biomedical Materials·R AkhtarN Gavara
Dec 13, 2016·Medical & Biological Engineering & Computing·Alireza Rezvani-SharifMaryam Sotoudeh-Anvari
Jan 10, 2018·Advances in Pharmacology·Jacob A VanderBurgh, Cynthia A Reinhart-King

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Citations

Jun 16, 2021·Molecular Biology Reports·María M Adeva-AndanyRaquel Funcasta-Calderón
Sep 10, 2021·Scientific Reports·Manon DouéStéphane Jaisson

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