Mechanical cues modulate cellular uptake of nanoparticles in cancer via clathrin-mediated and caveolae-mediated endocytosis pathways

Nanomedicine
Xiao WeiQinglei Gao

Abstract

To investigate the influence of tissue mechanics on the cellular uptake efficiency of nanoparticles (NPs) in cancer. Collagen-coated polyacrylamide gels were prepared as model substrates. Coumarin 6-loaded poly(lactic-co-glycolic) acid micelles (C6-NPs) were prepared to investigate the cellular uptake of NPs. We demonstrated that substrate stiffness modulated the cellular uptake of NPs of cancer. Mechanistically, mechanical cues exerted influence on the clathrin-mediated endocytosis and caveolae-mediated endocytosis pathways, which mediated stiffness-regulated cellular uptake of NPs. Our findings shed light on the regulatory role of the mechanical cues on the cellular uptake of NPs and will facilitate the selection of clinical patients who might benefit from a given nanotherapy.

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Citations

Jan 14, 2021·DNA and Cell Biology·Maic Audo Eybi Mayer SihombingGuixue Wang
Nov 1, 2020·International Journal of Molecular Sciences·Parinaz SabourianAshok Kakkar
Nov 15, 2020·Nanomaterials·Indra Van ZundertSusana Rocha
Sep 21, 2021·Journal of Experimental & Clinical Cancer Research : CR·Xiao WeiQinglei Gao

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Methods Mentioned

BETA
atomic force microscopy
flow cytometry
transmission electron microscopy
dynamic light scattering
Dynamic
Transmission
Fluorescence
fluorescence microscopy
xenograft
xenografts

Software Mentioned

GraphPad Prism

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