PMID: 15220735Jun 29, 2004Paper

Mechanical damage to corneal stromal cells by epithelial scraping

Cornea
Jin Zhao, T Nagasaki

Abstract

Corneal epithelial scraping, a common clinical procedure, triggers a loss of underlying keratocytes. This study was conducted to examine whether the physical impact of epithelial scrape injury plays any role in the death of these cells. Epithelial debridement was carried out on the cornea of a freshly killed mouse either by mechanical scraping with a blunt spatula, as in the clinical scraping, or lifting by repeated touching with a gelatin-coated slide. Subsequently, nuclei, nuclear envelope, microtubules, microfilaments, and plasma membranes were examined with specific probes. Some corneas were fixed with alcohol before scrape injury. Fate of keratocytes after epithelial injury was investigated with isolated eyes ex vivo and living mice in vivo. Some of the procedures were performed with human donor corneas. Within seconds of, or possibly simultaneous to, mechanical epithelial scraping, nuclei of underlying keratocytes became grossly deformed to assume extremely stretched morphology, accompanied by destruction of microfilaments and microtubules as well as compromised plasma membranes. These cells deteriorated gradually over several hours both ex vivo and in vivo. Nuclear deformation was observed even when the cornea was fixed ...Continue Reading

References

Jun 1, 1989·Archives of Ophthalmology·K D HannaM Menasche
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May 30, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Nathan EfronPhilip B Morgan
Aug 9, 2003·Experimental Eye Research·Jin Zhao, Takayuki Nagasaki
Jun 1, 1962·The Journal of Cell Biology·H HERRMANN, P L LEBEAU

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Citations

Jul 1, 2013·Asia-Pacific Journal of Ophthalmology·Lindsay A McGrath, Graham A Lee
Aug 16, 2014·Veterinary Ophthalmology·Julie E HempsteadBrian C Gilger
Dec 12, 2012·Ultrastructural Pathology·Asadollah KatbabMasoomeh Eghtedari

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