Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
J A PanaderoS Lanceros-Mendez

Abstract

In tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the material due to the micropore structure of the macro-pore walls that act as stress concentration points. In this work, chondrogenic precursor cells have been seeded in poly-ε-caprolactone (PCL) scaffolds with fibrin and some were submitted to free swelling culture and others to cyclic loading in a bioreactor. After cell culture, all the samples were analyzed for fatigue behavior under repeated loading-unloading cycles. Moreover, some components of the extracellular matrix (ECM) were identified. No differences were observed between samples undergoing free swelling or bioreactor loading conditions, neither respect to matrix components nor to mechanical performance to fatigue. The ECM did not achieve the desired preponderance of collagen type II over collagen type I which is considered the main characteristic of hyaline cartilage ...Continue Reading

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Citations

Jul 15, 2015·Biomechanics and Modeling in Mechanobiology·V CorreiaS Lanceros-Méndez
Sep 15, 2020·Advanced Biosystems·N CastroS Lanceros-Mendez
Dec 26, 2018·Advanced Engineering Materials·Katie M YochamDavid Estrada
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Sarah DavisTosca Roncada

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