Mechanical heterogeneity of dentin at different length scales as determined by AFM phase contrast

Micron : the International Research and Review Journal for Microscopy
Luiz E BertassoniMichael Vincent Swain

Abstract

In this study we sought to gain insights of the structural and mechanical heterogeneity of dentin at different length scales. We compared four distinct demineralization protocols with respect to their ability to expose the periodic pattern of dentin collagen. Additionally, we analyzed the phase contrast resulting from AFM images obtained in tapping mode to interrogate the viscoelastic behavior and surface adhesion properties of peritubular and intertubular dentin, and partially demineralized dentin collagen fibrils, particularly with respect to their gap and overlap regions. Results demonstrated that all demineralization protocols exposed the gap and overlap zones of dentin collagen fibrils. Phase contrast analyses suggested that the intertubular dentin, where the organic matrix is concentrated, generated a higher phase contrast due a higher contribution of energy dissipation (damping) than the highly mineralized peritubular region. At increasing amplitudes, viscoelasticity appeared to play a more significant contribution to the phase contrast of the images of collagen fibrils. The overlap region yielded a greater phase contrast than the more elastic gap zones. In summary, our results contribute to the perspective that, at diff...Continue Reading

References

Feb 1, 1983·Journal of Dental Research·W Distler, A Kröncke
Jan 1, 1983·Archives of Oral Biology·D L Singer, I Kleinberg
May 1, 1995·Biophysical Journal·D J HulmesP Fratzl
May 30, 1998·Journal of Dentistry·G W MarshallM Balooch
Oct 19, 2000·European Journal of Oral Sciences·M K BorgströmG Svensäter
May 15, 2001·Journal of Oral Rehabilitation·K SaekiG W Marshall
Sep 10, 2002·Journal of Structural Biology·Stefan HabelitzGrayson W Marshall
Apr 2, 2003·Biophysical Journal·Thomas GutsmannPaul K Hansma
May 24, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J H KinneyG W Marshall
Aug 20, 2003·Journal of Biomedical Materials Research. Part a·Lorenzo BreschiManuela Lopes
Sep 27, 2005·Micron : the International Research and Review Journal for Microscopy·R K NallaR O Ritchie
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joseph P R O OrgelTim J Wess
Apr 20, 2007·Dental Materials : Official Publication of the Academy of Dental Materials·Lorenzo BreschiElettra De Stefano Dorigo
Aug 28, 2007·Journal of Dental Research·S HabelitzA Gruverman
May 10, 2008·Journal of Structural Biology·M BaloochG W Marshall
Mar 6, 2010·Scanning·L E Bertassoni, G W Marshall
Sep 21, 2010·Scanning·Luiz Eduardo BertassoniGrayson William Marshall
Apr 14, 2006·Nanotechnology·Nicolás F Martínez, Ricardo García
Sep 6, 2011·Micron : the International Research and Review Journal for Microscopy·Luiz Eduardo BertassoniMichael Vincent Swain
Mar 15, 2012·Acta Biomaterialia·Luiz E BertassoniMichael V Swain

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Citations

Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Andreas StylianouEleni Alexandratou
Oct 6, 2015·Australian Dental Journal·K StankoskaL E Bertassoni
Dec 9, 2016·Journal of Dental Research·A A Leme-KrausA K Bedran-Russo
Nov 1, 2016·Connective Tissue Research·Luiz E Bertassoni, Michael V Swain

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