Mechanical injury of explants from the articulating surface of the inner meniscus

Archives of Biochemistry and Biophysics
John D KisidayDavid D Frisbie

Abstract

Knee osteoarthritis is accelerated by damage to the meniscus, a fibrocartilage tissue that assists in load transmission. However, little is known about the mechanical or cellular response of the meniscus to injurious overloading. Here, in vitro studies explored injury to meniscal explants using a compressive overloading protocol that has been well characterized for articular cartilage. Cartilage samples were processed in parallel as a reference to the extensive literature on cartilage injury. Injured meniscal explants showed extensive cell death at the articulating surface but no gross tissue damage, while similar conditions of peak stress and strain resulted in cartilage surface fissures and cell death consistent with moderate overloading. Post-injury gene expression in meniscal explants indicated a decrease in seven of the nine catabolic and pro-inflammatory molecules surveyed, while cartilage experienced a downregulation in ADAMTS-5 and TNF-alpha only. These data demonstrated a resiliency of the meniscus to injury, and that an acute increase in catabolic activities is not necessarily a consequence of mechanical overloading.

References

May 1, 1983·The American Journal of Sports Medicine·S P Arnoczky, R F Warren
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 25, 2004·Osteoarthritis and Cartilage·Stacy M ImlerMarc E Levenston
Jun 28, 2005·Osteoarthritis and Cartilage·P C M VerdonkG Verbruggen

❮ Previous
Next ❯

Citations

Apr 29, 2015·Osteoarthritis and Cartilage·J F Nishimuta, M E Levenston
Feb 1, 2012·Osteoarthritis and Cartilage·J F Nishimuta, M E Levenston
Mar 4, 2015·Journal of Biomechanics·Amy L McNulty, Farshid Guilak
Apr 8, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Garrett A CoatneyTammy L Haut Donahue
Nov 9, 2011·Osteoarthritis and Cartilage·E S FullerJ Melrose
Jul 21, 2015·Journal of Animal Physiology and Animal Nutrition·T N Ross-JonesJ Black
May 11, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Alex E CookJames L Cook
Jun 14, 2018·Tissue Engineering. Part B, Reviews·Mingxue ChenQuanyi Guo
Jan 25, 2020·Cells·Umberto PolitoAlessia Di Giancamillo
Feb 24, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Gerardo E NarezTammy L Haut Donahue

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.