PMID: 9545088Apr 17, 1998Paper

Mechanical injury to neuronal/glial cultures in microplates: role of NMDA receptors and pH in secondary neuronal cell death

Journal of Neuroscience Research
A G MukhinAlan I Faden

Abstract

In vitro models of traumatic injury are useful adjuncts to animal models for studying mechanisms of post-traumatic cell death. Here we describe a new in vitro model in which reproducible levels of injury are delivered by a punch device that produces 28 parallel cuts in individual wells of 96-well microplates. Cell loss is measured by LDH assay or quantitative fluorometric assay for ethidium homodimer staining. Glial cultures show cell death restricted to the initial injury site, whereas neuronal/glial cultures demonstrate substantial spread of cell loss over time. We used this model to examine the role of pH and NMDA receptors in delayed post-traumatic injury. NMDA receptor blockade by dizocilpine (MK-801) or treatment with antisense oligodeoxynucleotides directed against NMDAR1 was neuroprotective. Decreased cell death was observed under acidic conditions whereas increased extracellular pH was associated with increased, MK-801 sensitive cell loss. Advantages of our model include: reproducible trauma induction; rapid measurements of cell injury; and use of 96-well microplates which reduce time and cost. This model appears to be well-suited for the study of selected mechanisms of post-traumatic neuronal injury as well as for scr...Continue Reading

Citations

May 3, 2011·Annual Review of Biomedical Engineering·Barclay MorrisonMartin L Yarmush
Apr 1, 2009·Journal of Neurotrauma·Susan MarguliesUNKNOWN Combination Therapies for Traumatic Brain Injury Workshop Leaders
Apr 29, 2009·Journal of Neurotrauma·Yung Chia ChenDavid F Meaney
Feb 11, 2000·Annals of the New York Academy of Sciences·F SalaW Young
Jan 22, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Marong FangShu Han
Feb 13, 2001·Experimental Neurology·V A MovsesyanA I Faden
Nov 6, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Susan M KnoblachAlan I Faden
Jul 10, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Genell D HiltonAlan I Faden
Jan 5, 2002·Mental Retardation and Developmental Disabilities Research Reviews·P M Lea, A I Faden
Aug 10, 2000·Journal of Neuroscience Research·Z KörnyeiE Madarász
Mar 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Alan I FadenAlan P Kozikowski
Jul 4, 2001·Journal of Neuroscience Research·J W AllenA I Faden
Jul 10, 2007·In Vitro Cellular & Developmental Biology. Animal·Ximena A Lanosa, Jorge A Colombo
Oct 14, 2011·Annals of Biomedical Engineering·Tapan P PatelDavid F Meaney
Oct 12, 1999·The American Journal of Physiology·A I FadenA P Kozikowski
Oct 11, 2017·Alternatives to Laboratory Animals : ATLA·Ashwin Kumaria
Jan 25, 2000·American Journal of Physiology. Cell Physiology·J LiuJ M Hallenbeck
Oct 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W AllenA I Faden
Apr 16, 2021·Frontiers in Computational Neuroscience·Rosalind E MottDavid F Meaney

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